The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle

<i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sabrina S. Greening, Ji Zhang, Anne C. Midwinter, David A. Wilkinson, Scott McDougall, M. Carolyn Gates, Nigel P. French
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> mastitis whole-genome sequencing antimicrobial resistance cattle Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4b927c8c312d4ffba56a7b5aa4f61c8f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4b927c8c312d4ffba56a7b5aa4f61c8f
record_format	dspace
spelling	oai:doaj.org-article:4b927c8c312d4ffba56a7b5aa4f61c8f2021-11-25T19:12:12ZThe Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle10.3390/vetsci81102872306-7381https://doaj.org/article/4b927c8c312d4ffba56a7b5aa4f61c8f2021-11-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/287https://doaj.org/toc/2306-7381<i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles of mastitis-causing <i>S. aureus</i>. Whole-genome sequencing was performed on 57 <i>S. aureus</i> isolates derived from cows with either clinical or subclinical mastitis from 17 dairy herds in New Zealand. The genetic relatedness between isolates was examined using the core single nucleotide polymorphism alignment whilst AMR and virulence genes were identified <i>in-silico</i>. The association between gene presence-absence and sequence type (ST), antimicrobial susceptibility and dry cow therapy treatment was investigated using Scoary. Altogether, eight STs were identified with 61.4% (35/57) belonging to ST-1. Furthermore, 14 AMR-associated genes and 76 virulence-associated genes were identified, with little genetic diversity between isolates belonging to the same ST. Several genes including <i>merR1</i> which is thought to play a role in ciprofloxacin-resistance were found to be significantly overrepresented in isolates sampled from herds using ampicillin/cloxacillin dry cow therapy. Overall, the presence of resistance genes remains low and current antimicrobial usage patterns do not appear to be driving AMR in <i>S. aureus</i> associated with bovine mastitis.Sabrina S. GreeningJi ZhangAnne C. MidwinterDavid A. WilkinsonScott McDougallM. Carolyn GatesNigel P. FrenchMDPI AGarticle<i>Staphylococcus aureus</i>mastitiswhole-genome sequencingantimicrobial resistancecattleVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 287, p 287 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> mastitis whole-genome sequencing antimicrobial resistance cattle Veterinary medicine SF600-1100
spellingShingle	<i>Staphylococcus aureus</i> mastitis whole-genome sequencing antimicrobial resistance cattle Veterinary medicine SF600-1100 Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
description	<i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles of mastitis-causing <i>S. aureus</i>. Whole-genome sequencing was performed on 57 <i>S. aureus</i> isolates derived from cows with either clinical or subclinical mastitis from 17 dairy herds in New Zealand. The genetic relatedness between isolates was examined using the core single nucleotide polymorphism alignment whilst AMR and virulence genes were identified <i>in-silico</i>. The association between gene presence-absence and sequence type (ST), antimicrobial susceptibility and dry cow therapy treatment was investigated using Scoary. Altogether, eight STs were identified with 61.4% (35/57) belonging to ST-1. Furthermore, 14 AMR-associated genes and 76 virulence-associated genes were identified, with little genetic diversity between isolates belonging to the same ST. Several genes including <i>merR1</i> which is thought to play a role in ciprofloxacin-resistance were found to be significantly overrepresented in isolates sampled from herds using ampicillin/cloxacillin dry cow therapy. Overall, the presence of resistance genes remains low and current antimicrobial usage patterns do not appear to be driving AMR in <i>S. aureus</i> associated with bovine mastitis.
format	article
author	Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French
author_facet	Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French
author_sort	Sabrina S. Greening
title	The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
title_short	The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
title_full	The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
title_fullStr	The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
title_full_unstemmed	The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
title_sort	genetic relatedness and antimicrobial resistance patterns of mastitis-causing <i>staphylococcus aureus</i> strains isolated from new zealand dairy cattle
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4b927c8c312d4ffba56a7b5aa4f61c8f
work_keys_str_mv	AT sabrinasgreening thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT jizhang thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT annecmidwinter thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT davidawilkinson thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT scottmcdougall thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT mcarolyngates thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT nigelpfrench thegeneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT sabrinasgreening geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT jizhang geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT annecmidwinter geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT davidawilkinson geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT scottmcdougall geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT mcarolyngates geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle AT nigelpfrench geneticrelatednessandantimicrobialresistancepatternsofmastitiscausingistaphylococcusaureusistrainsisolatedfromnewzealanddairycattle
_version_	1718410153784508416

The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle

Ejemplares similares