Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.

Patients with chronic kidney disease (CKD) have significantly increased morbidity and mortality resulting from infections and cardiovascular diseases. Since monocytes play an essential role in host immunity, this study was directed to explore the gene expression profile in order to identify differen...

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Autores principales:	Heevy Abdulkareem Musa Al-Chaqmaqchi, Ali Moshfegh, Elham Dadfar, Josefin Paulsson, Moustapha Hassan, Stefan H Jacobson, Joachim Lundahl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b9451f2413145afad9abea6b573f934
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spelling	oai:doaj.org-article:4b9451f2413145afad9abea6b573f9342021-11-18T09:03:21ZActivation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.1932-620310.1371/journal.pone.0068937https://doaj.org/article/4b9451f2413145afad9abea6b573f9342013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935909/?tool=EBIhttps://doaj.org/toc/1932-6203Patients with chronic kidney disease (CKD) have significantly increased morbidity and mortality resulting from infections and cardiovascular diseases. Since monocytes play an essential role in host immunity, this study was directed to explore the gene expression profile in order to identify differences in activated pathways in monocytes relevant to the pathophysiology of atherosclerosis and increased susceptibility to infections. Monocytes from CKD patients (stages 4 and 5, estimated GFR <20 ml/min/1.73 m(2)) and healthy donors were collected from peripheral blood. Microarray gene expression profile was performed and data were interpreted by GeneSpring software and by PANTHER tool. Western blot was done to validate the pathway members. The results demonstrated that 600 and 272 genes were differentially up- and down regulated respectively in the patient group. Pathways involved in the inflammatory response were highly expressed and the Wnt/β-catenin signaling pathway was the most significant pathway expressed in the patient group. Since this pathway has been attributed to a variety of inflammatory manifestations, the current findings may contribute to dysfunctional monocytes in CKD patients. Strategies to interfere with this pathway may improve host immunity and prevent cardiovascular complications in CKD patients.Heevy Abdulkareem Musa Al-ChaqmaqchiAli MoshfeghElham DadfarJosefin PaulssonMoustapha HassanStefan H JacobsonJoachim LundahlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68937 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heevy Abdulkareem Musa Al-Chaqmaqchi Ali Moshfegh Elham Dadfar Josefin Paulsson Moustapha Hassan Stefan H Jacobson Joachim Lundahl Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
description	Patients with chronic kidney disease (CKD) have significantly increased morbidity and mortality resulting from infections and cardiovascular diseases. Since monocytes play an essential role in host immunity, this study was directed to explore the gene expression profile in order to identify differences in activated pathways in monocytes relevant to the pathophysiology of atherosclerosis and increased susceptibility to infections. Monocytes from CKD patients (stages 4 and 5, estimated GFR <20 ml/min/1.73 m(2)) and healthy donors were collected from peripheral blood. Microarray gene expression profile was performed and data were interpreted by GeneSpring software and by PANTHER tool. Western blot was done to validate the pathway members. The results demonstrated that 600 and 272 genes were differentially up- and down regulated respectively in the patient group. Pathways involved in the inflammatory response were highly expressed and the Wnt/β-catenin signaling pathway was the most significant pathway expressed in the patient group. Since this pathway has been attributed to a variety of inflammatory manifestations, the current findings may contribute to dysfunctional monocytes in CKD patients. Strategies to interfere with this pathway may improve host immunity and prevent cardiovascular complications in CKD patients.
format	article
author	Heevy Abdulkareem Musa Al-Chaqmaqchi Ali Moshfegh Elham Dadfar Josefin Paulsson Moustapha Hassan Stefan H Jacobson Joachim Lundahl
author_facet	Heevy Abdulkareem Musa Al-Chaqmaqchi Ali Moshfegh Elham Dadfar Josefin Paulsson Moustapha Hassan Stefan H Jacobson Joachim Lundahl
author_sort	Heevy Abdulkareem Musa Al-Chaqmaqchi
title	Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
title_short	Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
title_full	Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
title_fullStr	Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
title_full_unstemmed	Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
title_sort	activation of wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4b9451f2413145afad9abea6b573f934
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Activation of Wnt/β-catenin pathway in monocytes derived from chronic kidney disease patients.

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