Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult

Abstract Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the stria...

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Autores principales:	Dominika Luptakova, Ladislav Baciak, Tomas Pluhacek, Anton Skriba, Blanka Sediva, Vladimir Havlicek, Ivo Juranek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b9726728d1547239d5aa7c67427ce46
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spelling	oai:doaj.org-article:4b9726728d1547239d5aa7c67427ce462021-12-02T15:08:05ZMembrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult10.1038/s41598-018-25088-22045-2322https://doaj.org/article/4b9726728d1547239d5aa7c67427ce462018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25088-2https://doaj.org/toc/2045-2322Abstract Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the striatal level, the mass spectrometry imaging revealed an aberrant plasma membrane distribution of Na+/K+ ions in the oedema-affected areas. The failure of the Na+/K+ gradients was also apparent in the magnetic resonance imaging measurements, demonstrating intracellular water accumulation during the acute phase of the HI insult. During the subacute phase, compared with the control brains, an incipient accumulation of an array of N-acylphosphatidylethanolamine (NAPE) molecules was detected in the HI-affected brains, and both the cytotoxic and vasogenic types of oedema were detected. In the severely affected brain areas, abnormal distributions of the monosialogangliosides GM2 and GM3 were observed in two-thirds of the animals exposed to the insult. During the late stage, a partial restoration of the brain tissue was observed in most rats in both the in vivo and ex vivo studies. These specific molecular changes may be further utilized in neonatology practice in proposing and testing novel therapeutic strategies for the treatment of neonatal HI encephalopathy.Dominika LuptakovaLadislav BaciakTomas PluhacekAnton SkribaBlanka SedivaVladimir HavlicekIvo JuranekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominika Luptakova Ladislav Baciak Tomas Pluhacek Anton Skriba Blanka Sediva Vladimir Havlicek Ivo Juranek Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
description	Abstract Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the striatal level, the mass spectrometry imaging revealed an aberrant plasma membrane distribution of Na+/K+ ions in the oedema-affected areas. The failure of the Na+/K+ gradients was also apparent in the magnetic resonance imaging measurements, demonstrating intracellular water accumulation during the acute phase of the HI insult. During the subacute phase, compared with the control brains, an incipient accumulation of an array of N-acylphosphatidylethanolamine (NAPE) molecules was detected in the HI-affected brains, and both the cytotoxic and vasogenic types of oedema were detected. In the severely affected brain areas, abnormal distributions of the monosialogangliosides GM2 and GM3 were observed in two-thirds of the animals exposed to the insult. During the late stage, a partial restoration of the brain tissue was observed in most rats in both the in vivo and ex vivo studies. These specific molecular changes may be further utilized in neonatology practice in proposing and testing novel therapeutic strategies for the treatment of neonatal HI encephalopathy.
format	article
author	Dominika Luptakova Ladislav Baciak Tomas Pluhacek Anton Skriba Blanka Sediva Vladimir Havlicek Ivo Juranek
author_facet	Dominika Luptakova Ladislav Baciak Tomas Pluhacek Anton Skriba Blanka Sediva Vladimir Havlicek Ivo Juranek
author_sort	Dominika Luptakova
title	Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
title_short	Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
title_full	Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
title_fullStr	Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
title_full_unstemmed	Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
title_sort	membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4b9726728d1547239d5aa7c67427ce46
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Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult

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