Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer

Abstract Most bacteria release extracellular vesicles (EVs). Recent studies have found these vesicles are capable of gene delivery, however the consequences of vesicle-mediated transfer on the patterns and rates of gene flow within microbial communities remains unclear. Previous studies have not det...

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Autores principales:	Frances Tran, James Q. Boedicker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b976c58c7f8476a8b45465c5138eeaa
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spelling	oai:doaj.org-article:4b976c58c7f8476a8b45465c5138eeaa2021-12-02T12:32:54ZGenetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer10.1038/s41598-017-07447-72045-2322https://doaj.org/article/4b976c58c7f8476a8b45465c5138eeaa2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07447-7https://doaj.org/toc/2045-2322Abstract Most bacteria release extracellular vesicles (EVs). Recent studies have found these vesicles are capable of gene delivery, however the consequences of vesicle-mediated transfer on the patterns and rates of gene flow within microbial communities remains unclear. Previous studies have not determined the impact of both the genetic cargo and the donor and recipient species on the rate of vesicle-mediated gene exchange. This report examines the potential for EVs as a mechanism of gene transfer within heterogeneous microbial populations. EVs were harvested from three species of Gram-negative microbes carrying different plasmids. The dynamics of gene transfer into recipient species was measured. This study demonstrates that vesicles enable gene exchange between five species of Gram-negative bacteria, and that the identity of the genetic cargo, donor strain, and recipient strain all influence gene transfer rates. Each species released and acquired vesicles containing genetic material to a variable degree, and the transfer rate did not correlate with the relatedness of the donor and recipient species. The results suggest that EVs may be a general mechanism to exchange non-specialized genetic cargo between bacterial species.Frances TranJames Q. BoedickerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frances Tran James Q. Boedicker Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
description	Abstract Most bacteria release extracellular vesicles (EVs). Recent studies have found these vesicles are capable of gene delivery, however the consequences of vesicle-mediated transfer on the patterns and rates of gene flow within microbial communities remains unclear. Previous studies have not determined the impact of both the genetic cargo and the donor and recipient species on the rate of vesicle-mediated gene exchange. This report examines the potential for EVs as a mechanism of gene transfer within heterogeneous microbial populations. EVs were harvested from three species of Gram-negative microbes carrying different plasmids. The dynamics of gene transfer into recipient species was measured. This study demonstrates that vesicles enable gene exchange between five species of Gram-negative bacteria, and that the identity of the genetic cargo, donor strain, and recipient strain all influence gene transfer rates. Each species released and acquired vesicles containing genetic material to a variable degree, and the transfer rate did not correlate with the relatedness of the donor and recipient species. The results suggest that EVs may be a general mechanism to exchange non-specialized genetic cargo between bacterial species.
format	article
author	Frances Tran James Q. Boedicker
author_facet	Frances Tran James Q. Boedicker
author_sort	Frances Tran
title	Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
title_short	Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
title_full	Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
title_fullStr	Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
title_full_unstemmed	Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
title_sort	genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4b976c58c7f8476a8b45465c5138eeaa
work_keys_str_mv	AT francestran geneticcargoandbacterialspeciessettherateofvesiclemediatedhorizontalgenetransfer AT jamesqboedicker geneticcargoandbacterialspeciessettherateofvesiclemediatedhorizontalgenetransfer
_version_	1718393915176910848

Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer

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