Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.

<h4>Background</h4>Diagnosis of tuberculosis (TB) is still difficult. The purpose of our study was to evaluate the diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA (cfDNA) for diagnosing of TB.<h4>Methods</h4>We searched relevant databases for studies that used...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guocan Yu, Yanqin Shen, Bo Ye, Yan Shi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b9c333d75c64d01b258e7a24dc71e08
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4b9c333d75c64d01b258e7a24dc71e08
record_format	dspace
spelling	oai:doaj.org-article:4b9c333d75c64d01b258e7a24dc71e082021-12-02T20:10:10ZDiagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.1932-620310.1371/journal.pone.0253658https://doaj.org/article/4b9c333d75c64d01b258e7a24dc71e082021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253658https://doaj.org/toc/1932-6203<h4>Background</h4>Diagnosis of tuberculosis (TB) is still difficult. The purpose of our study was to evaluate the diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA (cfDNA) for diagnosing of TB.<h4>Methods</h4>We searched relevant databases for studies that used cfDNA to diagnose TB. We evaluated the accuracy of cfDNA compared with the composite reference standard (CRS) and culture. True positive, false positive, false negative, and true negative values for cfDNA were obtained first, then the estimated pooled sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic odds ratio (DOR), and the area under the summary receiver operating characteristic (SROC) curve (AUC) of cfDNA for diagnosing TB were calculated with 95% confidence intervals (CIs). Heterogeneity was determined using the I2 statistic. When the heterogeneity was obvious, the source of heterogeneity was further discussed.<h4>Results</h4>We included 14 independent studies comparing cfDNA with the CRS, and 4 studies compared with culture. The pooled sensitivity, specificity, PPV, NPV, DOR, and AUC of the SROC were 68%, 98%,99%, 62%, 83, and 0.97 as compared with the CRS, respectively. The pooled sensitivity, specificity, PPV, NPV, DOR, and AUC of the SROC were 48%, 91%, 92%, 60%, 5, and 0.88 as compared with culture, respectively. The heterogeneity between studies was significant.<h4>Conclusions</h4>The accuracy of cfDNA testing for TB diagnosis was good compared with CRS and culture. cfDNA can be used for rapid early diagnosis of TB.Guocan YuYanqin ShenBo YeYan ShiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253658 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guocan Yu Yanqin Shen Bo Ye Yan Shi Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
description	<h4>Background</h4>Diagnosis of tuberculosis (TB) is still difficult. The purpose of our study was to evaluate the diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA (cfDNA) for diagnosing of TB.<h4>Methods</h4>We searched relevant databases for studies that used cfDNA to diagnose TB. We evaluated the accuracy of cfDNA compared with the composite reference standard (CRS) and culture. True positive, false positive, false negative, and true negative values for cfDNA were obtained first, then the estimated pooled sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic odds ratio (DOR), and the area under the summary receiver operating characteristic (SROC) curve (AUC) of cfDNA for diagnosing TB were calculated with 95% confidence intervals (CIs). Heterogeneity was determined using the I2 statistic. When the heterogeneity was obvious, the source of heterogeneity was further discussed.<h4>Results</h4>We included 14 independent studies comparing cfDNA with the CRS, and 4 studies compared with culture. The pooled sensitivity, specificity, PPV, NPV, DOR, and AUC of the SROC were 68%, 98%,99%, 62%, 83, and 0.97 as compared with the CRS, respectively. The pooled sensitivity, specificity, PPV, NPV, DOR, and AUC of the SROC were 48%, 91%, 92%, 60%, 5, and 0.88 as compared with culture, respectively. The heterogeneity between studies was significant.<h4>Conclusions</h4>The accuracy of cfDNA testing for TB diagnosis was good compared with CRS and culture. cfDNA can be used for rapid early diagnosis of TB.
format	article
author	Guocan Yu Yanqin Shen Bo Ye Yan Shi
author_facet	Guocan Yu Yanqin Shen Bo Ye Yan Shi
author_sort	Guocan Yu
title	Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
title_short	Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
title_full	Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
title_fullStr	Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
title_full_unstemmed	Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.
title_sort	diagnostic accuracy of mycobacterium tuberculosis cell-free dna for tuberculosis: a systematic review and meta-analysis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4b9c333d75c64d01b258e7a24dc71e08
work_keys_str_mv	AT guocanyu diagnosticaccuracyofmycobacteriumtuberculosiscellfreednafortuberculosisasystematicreviewandmetaanalysis AT yanqinshen diagnosticaccuracyofmycobacteriumtuberculosiscellfreednafortuberculosisasystematicreviewandmetaanalysis AT boye diagnosticaccuracyofmycobacteriumtuberculosiscellfreednafortuberculosisasystematicreviewandmetaanalysis AT yanshi diagnosticaccuracyofmycobacteriumtuberculosiscellfreednafortuberculosisasystematicreviewandmetaanalysis
_version_	1718374997801566208

Diagnostic accuracy of Mycobacterium tuberculosis cell-free DNA for tuberculosis: A systematic review and meta-analysis.

Ejemplares similares