Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model

Abstract While the use of local anesthesia as part of multimodal pain management is common practice in human and veterinarian surgery, these drugs are not applied routinely in rodent surgery. Several recommendations on the use of local anesthesia exist, but systematic studies on their efficacy and s...

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Autores principales:	Mattea S. Durst, Margarete Arras, Rupert Palme, Steven R. Talbot, Paulin Jirkof
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b9c9702432e4c7a948890367209718d
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spelling	oai:doaj.org-article:4b9c9702432e4c7a948890367209718d2021-12-02T16:53:01ZLidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model10.1038/s41598-021-90331-22045-2322https://doaj.org/article/4b9c9702432e4c7a948890367209718d2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90331-2https://doaj.org/toc/2045-2322Abstract While the use of local anesthesia as part of multimodal pain management is common practice in human and veterinarian surgery, these drugs are not applied routinely in rodent surgery. Several recommendations on the use of local anesthesia exist, but systematic studies on their efficacy and side effects are lacking. In the present study, male and female C57BL/6J mice were subjected to a sham vasectomy or a sham embryo transfer, respectively. We tested whether a mixture of subcutaneously injected Lidocaine and Bupivacaine in combination with systemic Paracetamol applied via drinking water results in superior pain relief when compared to treatment with local anesthesia or Paracetamol alone. We applied a combination of methods to assess behavioral, emotional, and physiological changes indicative of pain. Voluntary Paracetamol intake via drinking water reached the target dosage of 200 mg/kg in most animals. Local anesthesia did not lead to obvious side effects such as irregular wound healing or systemic disorders. No relevant sex differences were detected in our study. Sevoflurane anesthesia and surgery affected physiological and behavioral measurements. Surprisingly, Paracetamol treatment alone significantly increased the Mouse Grimace Scale. Taken together, mice treated with a combination of local anesthesia and systemic analgesia did not show fewer signs of post-surgical pain or improved recovery compared to animals treated with either local anesthesia or Paracetamol.Mattea S. DurstMargarete ArrasRupert PalmeSteven R. TalbotPaulin JirkofNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mattea S. Durst Margarete Arras Rupert Palme Steven R. Talbot Paulin Jirkof Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
description	Abstract While the use of local anesthesia as part of multimodal pain management is common practice in human and veterinarian surgery, these drugs are not applied routinely in rodent surgery. Several recommendations on the use of local anesthesia exist, but systematic studies on their efficacy and side effects are lacking. In the present study, male and female C57BL/6J mice were subjected to a sham vasectomy or a sham embryo transfer, respectively. We tested whether a mixture of subcutaneously injected Lidocaine and Bupivacaine in combination with systemic Paracetamol applied via drinking water results in superior pain relief when compared to treatment with local anesthesia or Paracetamol alone. We applied a combination of methods to assess behavioral, emotional, and physiological changes indicative of pain. Voluntary Paracetamol intake via drinking water reached the target dosage of 200 mg/kg in most animals. Local anesthesia did not lead to obvious side effects such as irregular wound healing or systemic disorders. No relevant sex differences were detected in our study. Sevoflurane anesthesia and surgery affected physiological and behavioral measurements. Surprisingly, Paracetamol treatment alone significantly increased the Mouse Grimace Scale. Taken together, mice treated with a combination of local anesthesia and systemic analgesia did not show fewer signs of post-surgical pain or improved recovery compared to animals treated with either local anesthesia or Paracetamol.
format	article
author	Mattea S. Durst Margarete Arras Rupert Palme Steven R. Talbot Paulin Jirkof
author_facet	Mattea S. Durst Margarete Arras Rupert Palme Steven R. Talbot Paulin Jirkof
author_sort	Mattea S. Durst
title	Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
title_short	Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
title_full	Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
title_fullStr	Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
title_full_unstemmed	Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model
title_sort	lidocaine and bupivacaine as part of multimodal pain management in a c57bl/6j laparotomy mouse model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b9c9702432e4c7a948890367209718d
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Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model

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