FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.

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Detalles Bibliográficos
Autores principales:	Srinjan Basu, Lisa-Maria Needham, David Lando, Edward J. R. Taylor, Kai J. Wohlfahrt, Devina Shah, Wayne Boucher, Yi Lei Tan, Lawrence E. Bates, Olga Tkachenko, Julie Cramard, B. Christoffer Lagerholm, Christian Eggeling, Brian Hendrich, Dave Klenerman, Steven F. Lee, Ernest D. Laue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4ba0bd5ae2024d44b50386dc802df3dc
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spelling	oai:doaj.org-article:4ba0bd5ae2024d44b50386dc802df3dc2021-12-02T14:38:47ZFRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells10.1038/s41467-018-04486-02041-1723https://doaj.org/article/4ba0bd5ae2024d44b50386dc802df3dc2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04486-0https://doaj.org/toc/2041-1723Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.Srinjan BasuLisa-Maria NeedhamDavid LandoEdward J. R. TaylorKai J. WohlfahrtDevina ShahWayne BoucherYi Lei TanLawrence E. BatesOlga TkachenkoJulie CramardB. Christoffer LagerholmChristian EggelingBrian HendrichDave KlenermanSteven F. LeeErnest D. LaueNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
description	Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.
format	article
author	Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue
author_facet	Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue
author_sort	Srinjan Basu
title	FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
title_short	FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
title_full	FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
title_fullStr	FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
title_full_unstemmed	FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
title_sort	fret-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4ba0bd5ae2024d44b50386dc802df3dc
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FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

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