Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach
Hookworm is one type of soil-transmitted helminth, which could exert an anti-inflammatory effect in human or animal host, which provides a beneficial possibility for the discovery of inflammatory-related disease interventions. The identification of hookworm-derived anti-inflammatory molecules is urg...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:4ba0bfe7043f4d169b1c2e6954f2f5242021-11-11T07:42:23ZMining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach2235-298810.3389/fcimb.2021.781132https://doaj.org/article/4ba0bfe7043f4d169b1c2e6954f2f5242021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.781132/fullhttps://doaj.org/toc/2235-2988Hookworm is one type of soil-transmitted helminth, which could exert an anti-inflammatory effect in human or animal host, which provides a beneficial possibility for the discovery of inflammatory-related disease interventions. The identification of hookworm-derived anti-inflammatory molecules is urgently needed for future translational research. The emergence of metabolomics has become a powerful approach to comprehensively characterize metabolic alterations in recent times. Herein, excretory and secretory products (ESPs) were collected from cultured adult worm, while small intestinal contents were obtained from Nippostrongylus brasiliensis (N. brasiliensis, Nb)-infected mice. Through ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) platform, metabolomics analysis was used to explore the identification of anti-inflammatory molecules. Out of 45 differential metabolites that were discovered from ESPs, 10 of them showed potential anti-inflammatory properties, which could be subclassed into amino acids, furanocoumarins, linear diarylheptanoids, gamma butyrolactones, and alpha-keto acids. In terms of intestinal contents that were derived from N. brasiliensis-infected mice, 14 out of 301 differential metabolites were discovered to demonstrate anti-inflammatory effects, with possible subclassification into amino acids, benzylisoquinolines, quaternary ammonium salts, pyrimidines, pregnane steroids, purines, biphenyls, and glycerophosphocholines. Furthermore, nine of the differential metabolites appeared both in ESPs and infected intestinal contents, wherein four were proven to show anti-inflammation properties, namely, L-glutamine, glutamine (Gln), pyruvate, and alanine-Gln (Ala-Gln). In summary, we have provided a method for the identification and analysis of parasite-derived molecules with potential anti-inflammatory properties in the present study. This array of anti-inflammatory metabolites could provide clues for future evaluation and translational study of these anti-inflammatory molecules.Yuying ChenYuying ChenMingming ZhangXin DingYougui YangYougui YangYujia ChenQiang ZhangYinwen FanYang DaiYang DaiJunhong WangJunhong WangFrontiers Media S.A.articlehookwormNippostrongylus brasiliensisanti-inflammatory activityhost–pathogen interactionsmetabolomics approachMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
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hookworm Nippostrongylus brasiliensis anti-inflammatory activity host–pathogen interactions metabolomics approach Microbiology QR1-502 |
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hookworm Nippostrongylus brasiliensis anti-inflammatory activity host–pathogen interactions metabolomics approach Microbiology QR1-502 Yuying Chen Yuying Chen Mingming Zhang Xin Ding Yougui Yang Yougui Yang Yujia Chen Qiang Zhang Yinwen Fan Yang Dai Yang Dai Junhong Wang Junhong Wang Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| description |
Hookworm is one type of soil-transmitted helminth, which could exert an anti-inflammatory effect in human or animal host, which provides a beneficial possibility for the discovery of inflammatory-related disease interventions. The identification of hookworm-derived anti-inflammatory molecules is urgently needed for future translational research. The emergence of metabolomics has become a powerful approach to comprehensively characterize metabolic alterations in recent times. Herein, excretory and secretory products (ESPs) were collected from cultured adult worm, while small intestinal contents were obtained from Nippostrongylus brasiliensis (N. brasiliensis, Nb)-infected mice. Through ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) platform, metabolomics analysis was used to explore the identification of anti-inflammatory molecules. Out of 45 differential metabolites that were discovered from ESPs, 10 of them showed potential anti-inflammatory properties, which could be subclassed into amino acids, furanocoumarins, linear diarylheptanoids, gamma butyrolactones, and alpha-keto acids. In terms of intestinal contents that were derived from N. brasiliensis-infected mice, 14 out of 301 differential metabolites were discovered to demonstrate anti-inflammatory effects, with possible subclassification into amino acids, benzylisoquinolines, quaternary ammonium salts, pyrimidines, pregnane steroids, purines, biphenyls, and glycerophosphocholines. Furthermore, nine of the differential metabolites appeared both in ESPs and infected intestinal contents, wherein four were proven to show anti-inflammation properties, namely, L-glutamine, glutamine (Gln), pyruvate, and alanine-Gln (Ala-Gln). In summary, we have provided a method for the identification and analysis of parasite-derived molecules with potential anti-inflammatory properties in the present study. This array of anti-inflammatory metabolites could provide clues for future evaluation and translational study of these anti-inflammatory molecules. |
| format |
article |
| author |
Yuying Chen Yuying Chen Mingming Zhang Xin Ding Yougui Yang Yougui Yang Yujia Chen Qiang Zhang Yinwen Fan Yang Dai Yang Dai Junhong Wang Junhong Wang |
| author_facet |
Yuying Chen Yuying Chen Mingming Zhang Xin Ding Yougui Yang Yougui Yang Yujia Chen Qiang Zhang Yinwen Fan Yang Dai Yang Dai Junhong Wang Junhong Wang |
| author_sort |
Yuying Chen |
| title |
Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| title_short |
Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| title_full |
Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| title_fullStr |
Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| title_full_unstemmed |
Mining Anti-Inflammation Molecules From Nippostrongylus brasiliensis-Derived Products Through the Metabolomics Approach |
| title_sort |
mining anti-inflammation molecules from nippostrongylus brasiliensis-derived products through the metabolomics approach |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/4ba0bfe7043f4d169b1c2e6954f2f524 |
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