Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers
Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion ima...
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2021
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oai:doaj.org-article:4ba5917e35394733b2e8d180cf88ea402021-11-25T17:58:09ZMultiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers10.3390/ijms2222125911422-00671661-6596https://doaj.org/article/4ba5917e35394733b2e8d180cf88ea402021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12591https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion imaging using the novel SYN1 tracer based on the fluorine-18 isotope. The NOD-SCID mice were injected intravenously with SYN1 or [<sup>18</sup>F] fluorodeoxyglucose ([<sup>18</sup>F]-FDG) radiotracers after induction of the MI. In all studies, the positron emission tomography–computed tomography (PET/CT) technique was used. To obtain hemodynamic data, mice were subjected to magnetic resonance imaging (MRI). Finally, the biodistribution of the SYN1 compound was performed using Wistar rat model. SYN1 showed normal accumulation in mouse and rat hearts, and MI hearts correctly indicated impaired cardiac segments when compared to [<sup>18</sup>F]-FDG uptake. In vivo PET/CT and MRI studies showed statistical convergence in terms of the size of the necrotic zone and cardiac function. This was further supported with RNAseq molecular analyses to correlate the candidate function genes’ expression, with <i>Serpinb1c</i>, <i>Tnc</i> and <i>Nupr1</i>, with <i>Trem2</i> and <i>Aldolase B</i> functional correlations showing statistical significance in both SYN1 and [<sup>18</sup>F]-FDG. Our manuscript presents a new fluorine-18-based perfusion radiotracer for PET/CT imaging that may have importance in clinical applications. Future research should focus on confirmation of the data elucidated here to prepare SYN1 for first-in-human trials.Tomasz Jan KolanowskiWeronika Wargocka-MatuszewskaAgnieszka ZimnaLukasz ChedaJoanna Zyprych-WalczakAnna RugowskaMonika DrabikMichał FiedorowiczSeweryn KrajewskiŁukasz SteczekCezary KozaneckiZbigniew RogulskiNatalia RozwadowskaMaciej KurpiszMDPI AGarticlefluorine radiotracersSYN1[<sup>18</sup>F]-FDGFDGpositron emission tomography–computed tomography (PET/CT)molecular profiling after myocardial infarctionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12591, p 12591 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
fluorine radiotracers SYN1 [<sup>18</sup>F]-FDG FDG positron emission tomography–computed tomography (PET/CT) molecular profiling after myocardial infarction Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
fluorine radiotracers SYN1 [<sup>18</sup>F]-FDG FDG positron emission tomography–computed tomography (PET/CT) molecular profiling after myocardial infarction Biology (General) QH301-705.5 Chemistry QD1-999 Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| description |
Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion imaging using the novel SYN1 tracer based on the fluorine-18 isotope. The NOD-SCID mice were injected intravenously with SYN1 or [<sup>18</sup>F] fluorodeoxyglucose ([<sup>18</sup>F]-FDG) radiotracers after induction of the MI. In all studies, the positron emission tomography–computed tomography (PET/CT) technique was used. To obtain hemodynamic data, mice were subjected to magnetic resonance imaging (MRI). Finally, the biodistribution of the SYN1 compound was performed using Wistar rat model. SYN1 showed normal accumulation in mouse and rat hearts, and MI hearts correctly indicated impaired cardiac segments when compared to [<sup>18</sup>F]-FDG uptake. In vivo PET/CT and MRI studies showed statistical convergence in terms of the size of the necrotic zone and cardiac function. This was further supported with RNAseq molecular analyses to correlate the candidate function genes’ expression, with <i>Serpinb1c</i>, <i>Tnc</i> and <i>Nupr1</i>, with <i>Trem2</i> and <i>Aldolase B</i> functional correlations showing statistical significance in both SYN1 and [<sup>18</sup>F]-FDG. Our manuscript presents a new fluorine-18-based perfusion radiotracer for PET/CT imaging that may have importance in clinical applications. Future research should focus on confirmation of the data elucidated here to prepare SYN1 for first-in-human trials. |
| format |
article |
| author |
Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz |
| author_facet |
Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz |
| author_sort |
Tomasz Jan Kolanowski |
| title |
Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_short |
Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_full |
Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_fullStr |
Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_full_unstemmed |
Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_sort |
multiparametric evaluation of post-mi small animal models using metabolic ([<sup>18</sup>f]fdg) and perfusion-based (syn1) heart viability tracers |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/4ba5917e35394733b2e8d180cf88ea40 |
| work_keys_str_mv |
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