Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane a...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/4ba740fe79374755b67ce428ea357b1e |
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| Sumario: | A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5 ng/mL in 0.01 M PBS (pH 7.4) and 0.5 ng/mL in pig urine. All results were obtained within 5 min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB. |
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