Rapid detection of penbutolol in pig urine using an immunochromatographic test strip

A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane a...

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Autores principales:	Wenliang Ge, Steven Suryoprabowo, Liguang Xu, Qiankun Zheng, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	β-blocker immunochromatographic assay monoclonal antibody penbutolol strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4ba740fe79374755b67ce428ea357b1e
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spelling	oai:doaj.org-article:4ba740fe79374755b67ce428ea357b1e2021-11-26T11:19:47ZRapid detection of penbutolol in pig urine using an immunochromatographic test strip0954-01051465-344310.1080/09540105.2018.1520203https://doaj.org/article/4ba740fe79374755b67ce428ea357b1e2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1520203https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5 ng/mL in 0.01 M PBS (pH 7.4) and 0.5 ng/mL in pig urine. All results were obtained within 5 min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB.Wenliang GeSteven SuryoprabowoLiguang XuQiankun ZhengHua KuangTaylor & Francis Grouparticleβ-blockerimmunochromatographic assaymonoclonal antibodypenbutololstrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1126-1136 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-blocker immunochromatographic assay monoclonal antibody penbutolol strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	β-blocker immunochromatographic assay monoclonal antibody penbutolol strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
description	A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5 ng/mL in 0.01 M PBS (pH 7.4) and 0.5 ng/mL in pig urine. All results were obtained within 5 min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB.
format	article
author	Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang
author_facet	Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang
author_sort	Wenliang Ge
title	Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
title_short	Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
title_full	Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
title_fullStr	Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
title_full_unstemmed	Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
title_sort	rapid detection of penbutolol in pig urine using an immunochromatographic test strip
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/4ba740fe79374755b67ce428ea357b1e
work_keys_str_mv	AT wenliangge rapiddetectionofpenbutololinpigurineusinganimmunochromatographicteststrip AT stevensuryoprabowo rapiddetectionofpenbutololinpigurineusinganimmunochromatographicteststrip AT liguangxu rapiddetectionofpenbutololinpigurineusinganimmunochromatographicteststrip AT qiankunzheng rapiddetectionofpenbutololinpigurineusinganimmunochromatographicteststrip AT huakuang rapiddetectionofpenbutololinpigurineusinganimmunochromatographicteststrip
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Rapid detection of penbutolol in pig urine using an immunochromatographic test strip

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