Genomic Regions Associated with Fusarium Wilt Resistance in Flax

Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about t...

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Autores principales:	Alexander Kanapin, Mikhail Bankin, Tatyana Rozhmina, Anastasia Samsonova, Maria Samsonova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fusarium wilt resistance flax GWAS immune response disease severity index Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4baa9d34b9bf4446b15b523e520f2742
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spelling	oai:doaj.org-article:4baa9d34b9bf4446b15b523e520f27422021-11-25T17:56:05ZGenomic Regions Associated with Fusarium Wilt Resistance in Flax10.3390/ijms2222123831422-00671661-6596https://doaj.org/article/4baa9d34b9bf4446b15b523e520f27422021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12383https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about the mechanisms of resistance to Fusarium infection in flax. As a first step to uncover the genetic factors associated with resistance to Fusarium wilt disease, we performed a genome-wide association study (GWAS) using 297 accessions from the collection of the Federal Research Centre of the Bast Fiber Crops, Torzhok, Russia. These genotypes were infected with a highly pathogenic <i>Fusarium oxysporum</i> f.sp. <i>lini</i> MI39 strain; the wilt symptoms were documented in the course of three successive years. Six different single-locus models implemented in GAPIT3 R package were applied to a selected subset of 72,526 SNPs. A total of 15 QTNs (Quantitative Trait Nucleotides) were detected during at least two years of observation, while eight QTNs were found during all three years of the experiment. Of these, ten QTNs occupied a region of 640 Kb at the start of chromosome 1, while the remaining QTNs mapped to chromosomes 8, 11 and 13. All stable QTNs demonstrate a statistically significant allelic effect across 3 years of the experiment. Importantly, several QTNs spanned regions that harbored genes involved in the pathogen recognition and plant immunity response, including the KIP1-like protein (<i>Lus10025717</i>) and NBS-LRR protein (<i>Lus10025852</i>). Our results provide novel insights into the genetic architecture of flax resistance to Fusarium wilt and pinpoint potential candidate genes for further in-depth studies.Alexander KanapinMikhail BankinTatyana RozhminaAnastasia SamsonovaMaria SamsonovaMDPI AGarticlefusarium wiltresistanceflaxGWASimmune responsedisease severity indexBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12383, p 12383 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fusarium wilt resistance flax GWAS immune response disease severity index Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	fusarium wilt resistance flax GWAS immune response disease severity index Biology (General) QH301-705.5 Chemistry QD1-999 Alexander Kanapin Mikhail Bankin Tatyana Rozhmina Anastasia Samsonova Maria Samsonova Genomic Regions Associated with Fusarium Wilt Resistance in Flax
description	Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about the mechanisms of resistance to Fusarium infection in flax. As a first step to uncover the genetic factors associated with resistance to Fusarium wilt disease, we performed a genome-wide association study (GWAS) using 297 accessions from the collection of the Federal Research Centre of the Bast Fiber Crops, Torzhok, Russia. These genotypes were infected with a highly pathogenic <i>Fusarium oxysporum</i> f.sp. <i>lini</i> MI39 strain; the wilt symptoms were documented in the course of three successive years. Six different single-locus models implemented in GAPIT3 R package were applied to a selected subset of 72,526 SNPs. A total of 15 QTNs (Quantitative Trait Nucleotides) were detected during at least two years of observation, while eight QTNs were found during all three years of the experiment. Of these, ten QTNs occupied a region of 640 Kb at the start of chromosome 1, while the remaining QTNs mapped to chromosomes 8, 11 and 13. All stable QTNs demonstrate a statistically significant allelic effect across 3 years of the experiment. Importantly, several QTNs spanned regions that harbored genes involved in the pathogen recognition and plant immunity response, including the KIP1-like protein (<i>Lus10025717</i>) and NBS-LRR protein (<i>Lus10025852</i>). Our results provide novel insights into the genetic architecture of flax resistance to Fusarium wilt and pinpoint potential candidate genes for further in-depth studies.
format	article
author	Alexander Kanapin Mikhail Bankin Tatyana Rozhmina Anastasia Samsonova Maria Samsonova
author_facet	Alexander Kanapin Mikhail Bankin Tatyana Rozhmina Anastasia Samsonova Maria Samsonova
author_sort	Alexander Kanapin
title	Genomic Regions Associated with Fusarium Wilt Resistance in Flax
title_short	Genomic Regions Associated with Fusarium Wilt Resistance in Flax
title_full	Genomic Regions Associated with Fusarium Wilt Resistance in Flax
title_fullStr	Genomic Regions Associated with Fusarium Wilt Resistance in Flax
title_full_unstemmed	Genomic Regions Associated with Fusarium Wilt Resistance in Flax
title_sort	genomic regions associated with fusarium wilt resistance in flax
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4baa9d34b9bf4446b15b523e520f2742
work_keys_str_mv	AT alexanderkanapin genomicregionsassociatedwithfusariumwiltresistanceinflax AT mikhailbankin genomicregionsassociatedwithfusariumwiltresistanceinflax AT tatyanarozhmina genomicregionsassociatedwithfusariumwiltresistanceinflax AT anastasiasamsonova genomicregionsassociatedwithfusariumwiltresistanceinflax AT mariasamsonova genomicregionsassociatedwithfusariumwiltresistanceinflax
_version_	1718411800482938880

Genomic Regions Associated with Fusarium Wilt Resistance in Flax

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