Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.

Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These a...

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Autores principales:	Mahendran Subramanian, Jenson Lim, Jon Dobson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4badb1d64f3d4993983ba3641fbe36cd
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spelling	oai:doaj.org-article:4badb1d64f3d4993983ba3641fbe36cd2021-11-18T09:01:50ZEnhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.1932-620310.1371/journal.pone.0069812https://doaj.org/article/4badb1d64f3d4993983ba3641fbe36cd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936108/?tool=EBIhttps://doaj.org/toc/1932-6203Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.Mahendran SubramanianJenson LimJon DobsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69812 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahendran Subramanian Jenson Lim Jon Dobson Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
description	Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.
format	article
author	Mahendran Subramanian Jenson Lim Jon Dobson
author_facet	Mahendran Subramanian Jenson Lim Jon Dobson
author_sort	Mahendran Subramanian
title	Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
title_short	Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
title_full	Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
title_fullStr	Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
title_full_unstemmed	Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
title_sort	enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4badb1d64f3d4993983ba3641fbe36cd
work_keys_str_mv	AT mahendransubramanian enhancednanomagneticgenetransfectionofhumanprenatalcardiacprogenitorcellsandadultcardiomyocytes AT jensonlim enhancednanomagneticgenetransfectionofhumanprenatalcardiacprogenitorcellsandadultcardiomyocytes AT jondobson enhancednanomagneticgenetransfectionofhumanprenatalcardiacprogenitorcellsandadultcardiomyocytes
_version_	1718421018899382272

Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.

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