Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.

<h4>Background and aims</h4>Anti-sperm antibodies in can markedly reduce the likelihood of natural conception. The etiology of this anti-sperm immunity in human females is unknown. We compared the cytokine response of peripheral blood mononuclear cells (PBMCs) from infertile patients wit...

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Autores principales:	Miloslav Kverka, Zdenka Ulcova-Gallova, Jirina Bartova, Jan Cibulka, Katarina Bibkova, Zdenka Micanova, Helena Tlaskalova-Hogenova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4bae241da2b44dceae5cbbda6e662060
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spelling	oai:doaj.org-article:4bae241da2b44dceae5cbbda6e6620602021-11-18T07:06:52ZSperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.1932-620310.1371/journal.pone.0044172https://doaj.org/article/4bae241da2b44dceae5cbbda6e6620602012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952917/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and aims</h4>Anti-sperm antibodies in can markedly reduce the likelihood of natural conception. The etiology of this anti-sperm immunity in human females is unknown. We compared the cytokine response of peripheral blood mononuclear cells (PBMCs) from infertile patients with or without anti-sperm antibodies (ASA) and fertile women.<h4>Methodology/principal findings</h4>We cultivated the PBMCs together with sperm antigens (whole cells or cell lysate), and screened the supernatants for 40 cytokines by antibody array. When stimulated with whole sperm cells, the PBMCs from patients with ASA produce less IL-3, IL-11, IL-13, ICAM-1, GCSF and more IL-2, IL-4 and IL-12p70 as compared to healthy women. PBMCs from patients with ASA produce typically less IL-13, IL-7, IL-17 and MIG, and more MIP-1β and IL-8, as compared to PBMCs from patients without ASA. In response to sperm cell lysate, PBMCs from infertile women without ASA respond initially by increase in production of growth factors (GCSF, GM-CSF and PDGF-BB) followed by increase in chemokines (e.g. IL-8, MCP-1 and MIP-1β).<h4>Conclusions</h4>Cellular immune responses to sperm antigens, measured by production of cytokines, differ among infertile women with ASA, infertile women without ASA and healthy women. This difference could play an important role in the initial steps of the infertility pathogenesis.Miloslav KverkaZdenka Ulcova-GallovaJirina BartovaJan CibulkaKatarina BibkovaZdenka MicanovaHelena Tlaskalova-HogenovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e44172 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miloslav Kverka Zdenka Ulcova-Gallova Jirina Bartova Jan Cibulka Katarina Bibkova Zdenka Micanova Helena Tlaskalova-Hogenova Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
description	<h4>Background and aims</h4>Anti-sperm antibodies in can markedly reduce the likelihood of natural conception. The etiology of this anti-sperm immunity in human females is unknown. We compared the cytokine response of peripheral blood mononuclear cells (PBMCs) from infertile patients with or without anti-sperm antibodies (ASA) and fertile women.<h4>Methodology/principal findings</h4>We cultivated the PBMCs together with sperm antigens (whole cells or cell lysate), and screened the supernatants for 40 cytokines by antibody array. When stimulated with whole sperm cells, the PBMCs from patients with ASA produce less IL-3, IL-11, IL-13, ICAM-1, GCSF and more IL-2, IL-4 and IL-12p70 as compared to healthy women. PBMCs from patients with ASA produce typically less IL-13, IL-7, IL-17 and MIG, and more MIP-1β and IL-8, as compared to PBMCs from patients without ASA. In response to sperm cell lysate, PBMCs from infertile women without ASA respond initially by increase in production of growth factors (GCSF, GM-CSF and PDGF-BB) followed by increase in chemokines (e.g. IL-8, MCP-1 and MIP-1β).<h4>Conclusions</h4>Cellular immune responses to sperm antigens, measured by production of cytokines, differ among infertile women with ASA, infertile women without ASA and healthy women. This difference could play an important role in the initial steps of the infertility pathogenesis.
format	article
author	Miloslav Kverka Zdenka Ulcova-Gallova Jirina Bartova Jan Cibulka Katarina Bibkova Zdenka Micanova Helena Tlaskalova-Hogenova
author_facet	Miloslav Kverka Zdenka Ulcova-Gallova Jirina Bartova Jan Cibulka Katarina Bibkova Zdenka Micanova Helena Tlaskalova-Hogenova
author_sort	Miloslav Kverka
title	Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
title_short	Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
title_full	Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
title_fullStr	Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
title_full_unstemmed	Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
title_sort	sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/4bae241da2b44dceae5cbbda6e662060
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Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies.

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