Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat

Gentamicin (GM) is an aminoglycoside antibiotic that possesses a wide range of anti-microbial activity. Currently, uses of gentamicin are narrowed due to it supposedly induces nephrotoxicity. Therefore, the aim of this study is to evaluate the possible nephron-protective effect of wheat germ oil (WG...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lamiaa O. Hafez, Fatma A. Ali, Ashraf A. El-Ghoneimy, Magdy I. Abdel-Aziz
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2019
Materias:	Atrophy Gentamicin Nephrotoxicity Oxidative stress Wheat Germ Oil (WGO). Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4bb0e400228e45a09c31adfc4bc5ffc4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4bb0e400228e45a09c31adfc4bc5ffc4
record_format	dspace
spelling	oai:doaj.org-article:4bb0e400228e45a09c31adfc4bc5ffc42021-12-02T02:58:20ZNephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat10.21608/svu.2019.235822535-18262535-1877https://doaj.org/article/4bb0e400228e45a09c31adfc4bc5ffc42019-01-01T00:00:00Zhttps://svu.journals.ekb.eg/article_23582.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877Gentamicin (GM) is an aminoglycoside antibiotic that possesses a wide range of anti-microbial activity. Currently, uses of gentamicin are narrowed due to it supposedly induces nephrotoxicity. Therefore, the aim of this study is to evaluate the possible nephron-protective effect of wheat germ oil (WGO), and its antioxidant potential against gentamicin-induced toxicity in Wistar albino rats. Forty rats were randomly assigned to four different groups (Ten animals each); Group I was administered normal saline and acts as a control group. Group II was received WGO at a dose of (3 mg/kg by stomach gavage) daily for the 15successive days. Group III was administered gentamicin at the dose of (100 mg/kg i.p.) daily for 10 successive days. Group IV was given WGO as group II and one hour latter rats were treated with gentamicin as in group III. Rats in group III showed significant increases (p≤0.05) in serum creatinine and blood urea nitrogen (BUN) as well as renal malondialdehyde (MDA) levels together with significant (p≤0.05) reduction in glutathione (GSH) level and catalase (CAT) and superoxide dismutase (SOD) activities. In rats of group IV, creatinine and BUN levels were significantly (p≤0.05) reduced. Furthermore, renal GSH level and CAT and SOD activities were significantly (p≤0.05) increased in comparison to group III. Histopathological examination revealed variable grades of renal tissue alterations ranged from moderate to severe degrees of glomerular atrophy, vascular congestion, hemorrhage, tubular dilatation, necrosis and hyalinization in group III. In contrast, renal tissue in rats of group IV revealed glomerular cellularity of control group, reduction of tubular injury, and decreasing of collagen deposition. Therefore, WGO can effectively decrease the GM-induced renal injury as monitored by lipid peroxidation and histopathological examination.Lamiaa O. HafezFatma A. Ali Ashraf A. El-GhoneimyMagdy I. Abdel-AzizSouth Valley UniversityarticleAtrophyGentamicinNephrotoxicityOxidative stressWheat Germ Oil (WGO).AgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 2, Iss 1, Pp 51-67 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Atrophy Gentamicin Nephrotoxicity Oxidative stress Wheat Germ Oil (WGO). Agriculture S Veterinary medicine SF600-1100
spellingShingle	Atrophy Gentamicin Nephrotoxicity Oxidative stress Wheat Germ Oil (WGO). Agriculture S Veterinary medicine SF600-1100 Lamiaa O. Hafez Fatma A. Ali Ashraf A. El-Ghoneimy Magdy I. Abdel-Aziz Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
description	Gentamicin (GM) is an aminoglycoside antibiotic that possesses a wide range of anti-microbial activity. Currently, uses of gentamicin are narrowed due to it supposedly induces nephrotoxicity. Therefore, the aim of this study is to evaluate the possible nephron-protective effect of wheat germ oil (WGO), and its antioxidant potential against gentamicin-induced toxicity in Wistar albino rats. Forty rats were randomly assigned to four different groups (Ten animals each); Group I was administered normal saline and acts as a control group. Group II was received WGO at a dose of (3 mg/kg by stomach gavage) daily for the 15successive days. Group III was administered gentamicin at the dose of (100 mg/kg i.p.) daily for 10 successive days. Group IV was given WGO as group II and one hour latter rats were treated with gentamicin as in group III. Rats in group III showed significant increases (p≤0.05) in serum creatinine and blood urea nitrogen (BUN) as well as renal malondialdehyde (MDA) levels together with significant (p≤0.05) reduction in glutathione (GSH) level and catalase (CAT) and superoxide dismutase (SOD) activities. In rats of group IV, creatinine and BUN levels were significantly (p≤0.05) reduced. Furthermore, renal GSH level and CAT and SOD activities were significantly (p≤0.05) increased in comparison to group III. Histopathological examination revealed variable grades of renal tissue alterations ranged from moderate to severe degrees of glomerular atrophy, vascular congestion, hemorrhage, tubular dilatation, necrosis and hyalinization in group III. In contrast, renal tissue in rats of group IV revealed glomerular cellularity of control group, reduction of tubular injury, and decreasing of collagen deposition. Therefore, WGO can effectively decrease the GM-induced renal injury as monitored by lipid peroxidation and histopathological examination.
format	article
author	Lamiaa O. Hafez Fatma A. Ali Ashraf A. El-Ghoneimy Magdy I. Abdel-Aziz
author_facet	Lamiaa O. Hafez Fatma A. Ali Ashraf A. El-Ghoneimy Magdy I. Abdel-Aziz
author_sort	Lamiaa O. Hafez
title	Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
title_short	Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
title_full	Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
title_fullStr	Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
title_full_unstemmed	Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat
title_sort	nephro-protective effect of wheat germ oil on gentamicin-induced acute nephrotoxicity in wistar albino rat
publisher	South Valley University
publishDate	2019
url	https://doaj.org/article/4bb0e400228e45a09c31adfc4bc5ffc4
work_keys_str_mv	AT lamiaaohafez nephroprotectiveeffectofwheatgermoilongentamicininducedacutenephrotoxicityinwistaralbinorat AT fatmaaali nephroprotectiveeffectofwheatgermoilongentamicininducedacutenephrotoxicityinwistaralbinorat AT ashrafaelghoneimy nephroprotectiveeffectofwheatgermoilongentamicininducedacutenephrotoxicityinwistaralbinorat AT magdyiabdelaziz nephroprotectiveeffectofwheatgermoilongentamicininducedacutenephrotoxicityinwistaralbinorat
_version_	1718402039835262976

Nephro-Protective Effect of Wheat Germ Oil on Gentamicin-Induced Acute Nephrotoxicity in Wistar Albino Rat

Ejemplares similares