Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.

There is growing evidence that mitochondrial dysfunction, and more specifically fatty acid β-oxidation impairment, is involved in the pathophysiology of non-alcoholic steatohepatitis (NASH). The goal of the present study was to achieve more understanding on the modification/s of carnitinepalmitoyltr...

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Autores principales:	Gaetano Serviddio, Anna M Giudetti, Francesco Bellanti, Paola Priore, Tiziana Rollo, Rosanna Tamborra, Luisa Siculella, Gianluigi Vendemiale, Emanuele Altomare, Gabriele V Gnoni
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4bbd3c3dfb5a4b44b0932b8fbc5f91f4
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spelling	oai:doaj.org-article:4bbd3c3dfb5a4b44b0932b8fbc5f91f42021-11-18T06:46:51ZOxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.1932-620310.1371/journal.pone.0024084https://doaj.org/article/4bbd3c3dfb5a4b44b0932b8fbc5f91f42011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21909411/?tool=EBIhttps://doaj.org/toc/1932-6203There is growing evidence that mitochondrial dysfunction, and more specifically fatty acid β-oxidation impairment, is involved in the pathophysiology of non-alcoholic steatohepatitis (NASH). The goal of the present study was to achieve more understanding on the modification/s of carnitinepalmitoyltransferase-I (CPT-I), the rate-limiting enzyme of the mitochondrial fatty acid β-oxidation, during steatohepatitis. A high fat/methionine-choline deficient (MCD) diet, administered for 4 weeks, was used to induce NASH in rats.We demonstrated that CPT-I activity decreased, to the same extent, both in isolated liver mitochondria and in digitonin-permeabilized hepatocytes from MCD-diet fed rats.At the same time, the rate of total fatty acid oxidation to CO(2) and ketone bodies, measured in isolated hepatocytes, was significantly lowered in treated animals when compared to controls. Finally, an increase in CPT-I mRNA abundance and protein content, together with a high level of CPT-I protein oxidation was observed in treated rats. A posttranslational modification of rat CPT-I during steatohepatitis has been here discussed.Gaetano ServiddioAnna M GiudettiFrancesco BellantiPaola PrioreTiziana RolloRosanna TamborraLuisa SiculellaGianluigi VendemialeEmanuele AltomareGabriele V GnoniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24084 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gaetano Serviddio Anna M Giudetti Francesco Bellanti Paola Priore Tiziana Rollo Rosanna Tamborra Luisa Siculella Gianluigi Vendemiale Emanuele Altomare Gabriele V Gnoni Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
description	There is growing evidence that mitochondrial dysfunction, and more specifically fatty acid β-oxidation impairment, is involved in the pathophysiology of non-alcoholic steatohepatitis (NASH). The goal of the present study was to achieve more understanding on the modification/s of carnitinepalmitoyltransferase-I (CPT-I), the rate-limiting enzyme of the mitochondrial fatty acid β-oxidation, during steatohepatitis. A high fat/methionine-choline deficient (MCD) diet, administered for 4 weeks, was used to induce NASH in rats.We demonstrated that CPT-I activity decreased, to the same extent, both in isolated liver mitochondria and in digitonin-permeabilized hepatocytes from MCD-diet fed rats.At the same time, the rate of total fatty acid oxidation to CO(2) and ketone bodies, measured in isolated hepatocytes, was significantly lowered in treated animals when compared to controls. Finally, an increase in CPT-I mRNA abundance and protein content, together with a high level of CPT-I protein oxidation was observed in treated rats. A posttranslational modification of rat CPT-I during steatohepatitis has been here discussed.
format	article
author	Gaetano Serviddio Anna M Giudetti Francesco Bellanti Paola Priore Tiziana Rollo Rosanna Tamborra Luisa Siculella Gianluigi Vendemiale Emanuele Altomare Gabriele V Gnoni
author_facet	Gaetano Serviddio Anna M Giudetti Francesco Bellanti Paola Priore Tiziana Rollo Rosanna Tamborra Luisa Siculella Gianluigi Vendemiale Emanuele Altomare Gabriele V Gnoni
author_sort	Gaetano Serviddio
title	Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
title_short	Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
title_full	Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
title_fullStr	Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
title_full_unstemmed	Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
title_sort	oxidation of hepatic carnitine palmitoyl transferase-i (cpt-i) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4bbd3c3dfb5a4b44b0932b8fbc5f91f4
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Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.

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