West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system

Abstract West Nile virus (WNV) is a neurotropic pathogen which causes zoonotic disease in humans. Recently, there have been an increasing number of infected cases and there are no clinically approved vaccines or effective drugs to treat WNV infections in humans. The purpose of this study was to faci...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Li, Le Ma, Li-Ping Guo, Xiao-Lei Wang, Jing-Wei Zhang, Zhi-Gao Bu, Rong-Hong Hua
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4bc319313f894d56afbdc1f9e1b047a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4bc319313f894d56afbdc1f9e1b047a6
record_format	dspace
spelling	oai:doaj.org-article:4bc319313f894d56afbdc1f9e1b047a62021-12-02T11:53:13ZWest Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system10.1038/s41598-017-03670-42045-2322https://doaj.org/article/4bc319313f894d56afbdc1f9e1b047a62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03670-4https://doaj.org/toc/2045-2322Abstract West Nile virus (WNV) is a neurotropic pathogen which causes zoonotic disease in humans. Recently, there have been an increasing number of infected cases and there are no clinically approved vaccines or effective drugs to treat WNV infections in humans. The purpose of this study was to facilitate vaccine and antiviral drug discovery by developing a packaging cell line-restricted WNV infectious replicon particle system. We constructed a DNA-based WNV replicon lacking the C-prM-E coding region and replaced it with a GFP coding sequence. To produce WNV replicon particles, cell lines stably-expressing prM-E and C-prM-E were constructed. When the WNV replicon plasmid was co-transfected with a WNV C-expressing plasmid into the prM-E-expressing cell line or directly transfected the C-prM-E expressing cell line, the replicon particle was able to replicate, form green fluorescence foci, and exhibit cytopathic plaques similar to that induced by the wild type virus. The infectious capacity of the replicon particles was restricted to the packaging cell line as the replicons demonstrated only one round of infection in other permissive cells. Thus, this system provides a safe and convenient reporter WNV manipulating tool which can be used to study WNV viral invasion mechanisms, neutralizing antibodies and antiviral efficacy.Wei LiLe MaLi-Ping GuoXiao-Lei WangJing-Wei ZhangZhi-Gao BuRong-Hong HuaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Li Le Ma Li-Ping Guo Xiao-Lei Wang Jing-Wei Zhang Zhi-Gao Bu Rong-Hong Hua West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
description	Abstract West Nile virus (WNV) is a neurotropic pathogen which causes zoonotic disease in humans. Recently, there have been an increasing number of infected cases and there are no clinically approved vaccines or effective drugs to treat WNV infections in humans. The purpose of this study was to facilitate vaccine and antiviral drug discovery by developing a packaging cell line-restricted WNV infectious replicon particle system. We constructed a DNA-based WNV replicon lacking the C-prM-E coding region and replaced it with a GFP coding sequence. To produce WNV replicon particles, cell lines stably-expressing prM-E and C-prM-E were constructed. When the WNV replicon plasmid was co-transfected with a WNV C-expressing plasmid into the prM-E-expressing cell line or directly transfected the C-prM-E expressing cell line, the replicon particle was able to replicate, form green fluorescence foci, and exhibit cytopathic plaques similar to that induced by the wild type virus. The infectious capacity of the replicon particles was restricted to the packaging cell line as the replicons demonstrated only one round of infection in other permissive cells. Thus, this system provides a safe and convenient reporter WNV manipulating tool which can be used to study WNV viral invasion mechanisms, neutralizing antibodies and antiviral efficacy.
format	article
author	Wei Li Le Ma Li-Ping Guo Xiao-Lei Wang Jing-Wei Zhang Zhi-Gao Bu Rong-Hong Hua
author_facet	Wei Li Le Ma Li-Ping Guo Xiao-Lei Wang Jing-Wei Zhang Zhi-Gao Bu Rong-Hong Hua
author_sort	Wei Li
title	West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
title_short	West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
title_full	West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
title_fullStr	West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
title_full_unstemmed	West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
title_sort	west nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4bc319313f894d56afbdc1f9e1b047a6
work_keys_str_mv	AT weili westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT lema westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT lipingguo westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT xiaoleiwang westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT jingweizhang westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT zhigaobu westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem AT ronghonghua westnilevirusinfectiousrepliconparticlesgeneratedusingapackagingrestrictedcelllineisasafereportersystem
_version_	1718394864369926144

West Nile virus infectious replicon particles generated using a packaging-restricted cell line is a safe reporter system

Ejemplares similares