Systematic detection of functional proteoform groups from bottom-up proteomic datasets

Many proteins exist in various proteoforms but detecting these variants by bottom-up proteomics remains difficult. Here, the authors present a computational approach based on peptide correlation analysis to identify and characterize proteoforms from bottom-up proteomics data.

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Bibliographic Details
Main Authors: Isabell Bludau, Max Frank, Christian Dörig, Yujia Cai, Moritz Heusel, George Rosenberger, Paola Picotti, Ben C. Collins, Hannes Röst, Ruedi Aebersold
Format: article
Language:EN
Published: Nature Portfolio 2021
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Q
Online Access:https://doaj.org/article/4bc622ef871944e7b4a94176e06f1144
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Summary:Many proteins exist in various proteoforms but detecting these variants by bottom-up proteomics remains difficult. Here, the authors present a computational approach based on peptide correlation analysis to identify and characterize proteoforms from bottom-up proteomics data.