Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot

Dried blood spots (DBS) have advantages such as minimizing blood collection volume and the distress to neonate. DBS have been used for tandem mass spectrometry (MS/MS)-based newborn screening tests (NST) of amino acid (AA) and acylcarnitine. The Newborn Screening Quality Assurance Program (NSQAP) ha...

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Autor principal:	Hyun-Seung Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	arginine dried blood spot amino acid acylcarnitine real-time stability accelerated degradation study Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4bc7c27b1f3a4ec7a8ff79617c4c18a3
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spelling	oai:doaj.org-article:4bc7c27b1f3a4ec7a8ff79617c4c18a32021-11-25T18:59:19ZArginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot10.3390/separations81102012297-8739https://doaj.org/article/4bc7c27b1f3a4ec7a8ff79617c4c18a32021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/201https://doaj.org/toc/2297-8739Dried blood spots (DBS) have advantages such as minimizing blood collection volume and the distress to neonate. DBS have been used for tandem mass spectrometry (MS/MS)-based newborn screening tests (NST) of amino acid (AA) and acylcarnitine. The Newborn Screening Quality Assurance Program (NSQAP) have been provided quality control (QC) materials for MS/MS, as DBS cards. The NSQAP is generally provided within 14 months of the shelf life and the recommended storage condition is at −10 °C to −30 °C. Previously, several accelerated degradation studies had been performed to determine the transportation stability and short-term stability of AAs and acylcarnitines in DBS. However, the experimental condition is markedly different to the storage condition. We performed long-term monitoring for the real-time stability of seven AAs and 14 acylcarnitines from three levels of 2012 NSQAP QC materials across a time period of 788 days. Arginine suddenly yielded a catastrophic degeneration pattern, which started around D300. When comparing this with previous accelerated degradation studies, methionine, tyrosine, citrulline, and acetylcarnitine did not show a remarkable measurand drift for the real-time stability, except for arginine. Our study showed that arginine would require intensive QC monitoring in routine practice, and should be used for the assessment of the stability in long-term storage of DBS samples for biobanking.Hyun-Seung LeeMDPI AGarticleargininedried blood spotamino acidacylcarnitinereal-time stabilityaccelerated degradation studyPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 201, p 201 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	arginine dried blood spot amino acid acylcarnitine real-time stability accelerated degradation study Physics QC1-999 Chemistry QD1-999
spellingShingle	arginine dried blood spot amino acid acylcarnitine real-time stability accelerated degradation study Physics QC1-999 Chemistry QD1-999 Hyun-Seung Lee Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
description	Dried blood spots (DBS) have advantages such as minimizing blood collection volume and the distress to neonate. DBS have been used for tandem mass spectrometry (MS/MS)-based newborn screening tests (NST) of amino acid (AA) and acylcarnitine. The Newborn Screening Quality Assurance Program (NSQAP) have been provided quality control (QC) materials for MS/MS, as DBS cards. The NSQAP is generally provided within 14 months of the shelf life and the recommended storage condition is at −10 °C to −30 °C. Previously, several accelerated degradation studies had been performed to determine the transportation stability and short-term stability of AAs and acylcarnitines in DBS. However, the experimental condition is markedly different to the storage condition. We performed long-term monitoring for the real-time stability of seven AAs and 14 acylcarnitines from three levels of 2012 NSQAP QC materials across a time period of 788 days. Arginine suddenly yielded a catastrophic degeneration pattern, which started around D300. When comparing this with previous accelerated degradation studies, methionine, tyrosine, citrulline, and acetylcarnitine did not show a remarkable measurand drift for the real-time stability, except for arginine. Our study showed that arginine would require intensive QC monitoring in routine practice, and should be used for the assessment of the stability in long-term storage of DBS samples for biobanking.
format	article
author	Hyun-Seung Lee
author_facet	Hyun-Seung Lee
author_sort	Hyun-Seung Lee
title	Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
title_short	Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
title_full	Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
title_fullStr	Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
title_full_unstemmed	Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
title_sort	arginine, as a key indicator for real-time stability monitoring of quality control in the newborn screening test using dried blood spot
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4bc7c27b1f3a4ec7a8ff79617c4c18a3
work_keys_str_mv	AT hyunseunglee arginineasakeyindicatorforrealtimestabilitymonitoringofqualitycontrolinthenewbornscreeningtestusingdriedbloodspot
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Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot

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