Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity

Abstract The voiding of urine has a clear circadian rhythm with increased voiding during active phases and decreased voiding during inactive phases. Bladder spinal afferents play a key role in the regulation of bladder storage and voiding, but it is unknown whether they exhibit themselves a potentia...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Stewart Christie, Vladimir Zagorodnyuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4bc815dfb87e4bc2b3a7e2da7f3ad38e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4bc815dfb87e4bc2b3a7e2da7f3ad38e
record_format	dspace
spelling	oai:doaj.org-article:4bc815dfb87e4bc2b3a7e2da7f3ad38e2021-12-02T17:37:12ZTime-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity10.1038/s41598-021-98831-x2045-2322https://doaj.org/article/4bc815dfb87e4bc2b3a7e2da7f3ad38e2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98831-xhttps://doaj.org/toc/2045-2322Abstract The voiding of urine has a clear circadian rhythm with increased voiding during active phases and decreased voiding during inactive phases. Bladder spinal afferents play a key role in the regulation of bladder storage and voiding, but it is unknown whether they exhibit themselves a potential circadian rhythm. Therefore, this study aimed to determine the mechano- and chemo- sensitivity of three major bladder afferent classes at two opposite day-night time points. Adult female guinea pigs underwent conscious voiding monitoring and bladder ex vivo single unit extracellular afferent recordings at 0300 h and 1500 h to determine day-night modulation of bladder afferent activity. All guinea pigs voided a higher amount of urine at 1500 h compared to 0300 h. This was due to an increased number of voids at 1500 h. The mechano-sensitivity of low- and high-threshold stretch-sensitive muscular-mucosal bladder afferents to mucosal stroking and stretch was significantly higher at 1500 h compared to 0300 h. Low-threshold stretch-insensitive mucosal afferent sensitivity to stroking was significantly higher at 1500 h compared to 0300 h. Further, the chemosensitivity of mucosal afferents to N-Oleoyl Dopamine (endogenous TRPV1 agonist) was also significantly increased at 1500 h compared to 0300 h. This data indicates that bladder afferents exhibit a significant time-of-day dependent variation in mechano-sensitivity which may influence urine voiding patterns. Further studies across a 24 h period are warranted to reveal potential circadian rhythm modulation of bladder afferent activity.Stewart ChristieVladimir ZagorodnyukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stewart Christie Vladimir Zagorodnyuk Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
description	Abstract The voiding of urine has a clear circadian rhythm with increased voiding during active phases and decreased voiding during inactive phases. Bladder spinal afferents play a key role in the regulation of bladder storage and voiding, but it is unknown whether they exhibit themselves a potential circadian rhythm. Therefore, this study aimed to determine the mechano- and chemo- sensitivity of three major bladder afferent classes at two opposite day-night time points. Adult female guinea pigs underwent conscious voiding monitoring and bladder ex vivo single unit extracellular afferent recordings at 0300 h and 1500 h to determine day-night modulation of bladder afferent activity. All guinea pigs voided a higher amount of urine at 1500 h compared to 0300 h. This was due to an increased number of voids at 1500 h. The mechano-sensitivity of low- and high-threshold stretch-sensitive muscular-mucosal bladder afferents to mucosal stroking and stretch was significantly higher at 1500 h compared to 0300 h. Low-threshold stretch-insensitive mucosal afferent sensitivity to stroking was significantly higher at 1500 h compared to 0300 h. Further, the chemosensitivity of mucosal afferents to N-Oleoyl Dopamine (endogenous TRPV1 agonist) was also significantly increased at 1500 h compared to 0300 h. This data indicates that bladder afferents exhibit a significant time-of-day dependent variation in mechano-sensitivity which may influence urine voiding patterns. Further studies across a 24 h period are warranted to reveal potential circadian rhythm modulation of bladder afferent activity.
format	article
author	Stewart Christie Vladimir Zagorodnyuk
author_facet	Stewart Christie Vladimir Zagorodnyuk
author_sort	Stewart Christie
title	Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
title_short	Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
title_full	Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
title_fullStr	Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
title_full_unstemmed	Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
title_sort	time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4bc815dfb87e4bc2b3a7e2da7f3ad38e
work_keys_str_mv	AT stewartchristie timeofdaydependentchangesinguineapigbladderafferentmechanosensitivity AT vladimirzagorodnyuk timeofdaydependentchangesinguineapigbladderafferentmechanosensitivity
_version_	1718379880985395200

Time-of-day dependent changes in guinea pig bladder afferent mechano-sensitivity

Ejemplares similares