Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities

Abstract. Akwu N, Naidoo Y, Singh M, Thimmegowda SC, Nundkumar N, Lin J. 2020. Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities. Biodiversitas 21: 5684-5691. Lupeol a pentacyclic triterpenoid, was extracted from the stem bark of Grewia las...

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Autores principales:	Nneka Akwu, Yougasphree Naidoo, Moganavelli Singh, Sadashiva Channangihalli Thimmegowda, Nirasha Nundkumar, Johnson Lin
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antibacterial, antioxidant, cytotoxicity, grewia lasiocarpa, lupeol, stem bark Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4bcb9eb2ee3b46b58a02ab54d78fbbda
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spelling	oai:doaj.org-article:4bcb9eb2ee3b46b58a02ab54d78fbbda2021-11-22T00:52:01ZIsolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities1412-033X2085-472210.13057/biodiv/d211213https://doaj.org/article/4bcb9eb2ee3b46b58a02ab54d78fbbda2020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6508https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Akwu N, Naidoo Y, Singh M, Thimmegowda SC, Nundkumar N, Lin J. 2020. Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities. Biodiversitas 21: 5684-5691. Lupeol a pentacyclic triterpenoid, was extracted from the stem bark of Grewia lasiocarpa. Stem bark was macerated with chloroform (CHCl3) at 25 ± 2°C, and then purified using a silica gel column. The mobile phase was hexane (100%) and hexane: ethyl acetate (80:20) as the mobile phase. The structure of lupeol was identified by 1HNMR, and 13C NMR spectral data, compared with reported data. The antibacterial, antioxidant and cytotoxicity activities of lupeol were evaluated using agar-well diffusion against six clinic bacteria isolates, namely Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 314588), and Salmonella typhimurium (ATCC 14026) Gram-positive bacteria: Staphylococcus aureus (ATCC 25923), methicillin-resistant Staphylococcus aureus (MRSA) (ATCC BAA-1683). The antioxidants assays were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). Cytotoxicity activity was carried out by MTT (3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide) assay was carried out against HEK293 (human embryonic kidney), HeLa (cervical carcinoma), and MCF-7 (breast adenocarcinoma) cell lines. The concentration range of lupeol was 240-15 µg/mL. Lupeol at the concentration of 240 and 120 µg/mL have no antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) (ATCC BAA-1683). The antioxidant activity was dose-dependent, while cytotoxic activities were observed in HEK293, HeLa, and MCF-7 cells. This study was the first report on the extraction, isolation, and biological activities of lupeol, from the stem bark of G. lasiocarpa.Nneka AkwuYougasphree NaidooMoganavelli SinghSadashiva Channangihalli ThimmegowdaNirasha NundkumarJohnson LinMBI & UNS Soloarticleantibacterial, antioxidant, cytotoxicity, grewia lasiocarpa, lupeol, stem barkBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial, antioxidant, cytotoxicity, grewia lasiocarpa, lupeol, stem bark Biology (General) QH301-705.5
spellingShingle	antibacterial, antioxidant, cytotoxicity, grewia lasiocarpa, lupeol, stem bark Biology (General) QH301-705.5 Nneka Akwu Yougasphree Naidoo Moganavelli Singh Sadashiva Channangihalli Thimmegowda Nirasha Nundkumar Johnson Lin Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
description	Abstract. Akwu N, Naidoo Y, Singh M, Thimmegowda SC, Nundkumar N, Lin J. 2020. Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities. Biodiversitas 21: 5684-5691. Lupeol a pentacyclic triterpenoid, was extracted from the stem bark of Grewia lasiocarpa. Stem bark was macerated with chloroform (CHCl3) at 25 ± 2°C, and then purified using a silica gel column. The mobile phase was hexane (100%) and hexane: ethyl acetate (80:20) as the mobile phase. The structure of lupeol was identified by 1HNMR, and 13C NMR spectral data, compared with reported data. The antibacterial, antioxidant and cytotoxicity activities of lupeol were evaluated using agar-well diffusion against six clinic bacteria isolates, namely Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 314588), and Salmonella typhimurium (ATCC 14026) Gram-positive bacteria: Staphylococcus aureus (ATCC 25923), methicillin-resistant Staphylococcus aureus (MRSA) (ATCC BAA-1683). The antioxidants assays were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). Cytotoxicity activity was carried out by MTT (3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide) assay was carried out against HEK293 (human embryonic kidney), HeLa (cervical carcinoma), and MCF-7 (breast adenocarcinoma) cell lines. The concentration range of lupeol was 240-15 µg/mL. Lupeol at the concentration of 240 and 120 µg/mL have no antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) (ATCC BAA-1683). The antioxidant activity was dose-dependent, while cytotoxic activities were observed in HEK293, HeLa, and MCF-7 cells. This study was the first report on the extraction, isolation, and biological activities of lupeol, from the stem bark of G. lasiocarpa.
format	article
author	Nneka Akwu Yougasphree Naidoo Moganavelli Singh Sadashiva Channangihalli Thimmegowda Nirasha Nundkumar Johnson Lin
author_facet	Nneka Akwu Yougasphree Naidoo Moganavelli Singh Sadashiva Channangihalli Thimmegowda Nirasha Nundkumar Johnson Lin
author_sort	Nneka Akwu
title	Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
title_short	Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
title_full	Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
title_fullStr	Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
title_full_unstemmed	Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities
title_sort	isolation of lupeol from grewia lasiocarpa stem bark: antibacterial, antioxidant, and cytotoxicity activities
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/4bcb9eb2ee3b46b58a02ab54d78fbbda
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Isolation of lupeol from Grewia lasiocarpa stem bark: Antibacterial, antioxidant, and cytotoxicity activities

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