Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats

The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains...

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Autores principales:	A. E. Goncharov, A. P. Solomenny, A. L. Panin, S. E. Grigoriev, M. Yu. Cheprasov, Ya. A. Ahremenko, V. V. Kolodzieva, N. E. Goncharov, L. A. Kraeva
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	serratia, biofilms, extreme environment, psychrotolerant bacteria, cold adaptation, virulence, microbiological monitoring Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6
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spelling	oai:doaj.org-article:4bcccb4db5c54f24b1e2fad6f8abced62021-11-22T07:09:55ZAssessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats2220-76192313-739810.15789/2220-7619-ASS-1593https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced62021-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1593https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains in this genus remains unexplored. The aim of this study was to compare the genomes of two Serratia strains isolated in polar regions, primarily examining genetic factors of virulence and adaptation to cryogenic environment. During the 56th Russian Antarctic Expedition the Serratia liquefaciens 72 strain was isolated from a guano sample of the Adelie Penguin (Pygoscelis adeliae) colony on Tokarev Island (Haswell Archipelago, East Antarctica). The Serratia fonticola 5l strain was isolated from the frozen carcass of moose (Alces alces) fossils found on the Buor-Khaya Peninsula near the Laptev Sea coast (Yakutia Region, Russia). The whole-genome sequencing of such strains allowed to reveal genetic structures evidencing about their successful adaptation to low temperatures. Thus, it was found that both genomes contain genes encoding the main cold shock proteins, phylogenetically close to the corresponding genes in the hypobarotolerant Serratia liquefaciens strain ATCC 27592. Furthermore, both strains bear a cluster of tc-fABCD genes determining the bacterial adhesion to epithelial tissues, and the genes for RTX toxins — adhesins, crucial factors of biofilm formation in pathogenic Gram-negative bacteria. Experimental studies confirmed the ability of Serratia liquefaciens 72 and Serratia fonticola 5l to actively form biofilms in a wide temperature range (from 6°C to 37°C). The results obtained indicate that the examined genus Serratia strains isolated in Arctica and Antarctica exert overall similar adaptation strategies to polar climate, including the ability to produce pili, show active adhesion, and biofilm formation under low temperatures. Genetic adaptive factors may also act as pathogenicity factors allowing extremotolerant Serratia strains to exert traits of opportunistic and nosocomial pathogens and spread via chilled food-borne transmission. The wide use of food technologies, such as cooling and vacuum sealing, can potentially create a new ecological niche favourable for selection of psychrotolerant and hypobarotolerant pathogens. The data obtained allow to raise a question about necessity of further studies to monitor genetic diversity among psychrophilic hypobarotolerant microbial populations possessing pathogenic and epidemic potential.A. E. GoncharovA. P. SolomennyA. L. PaninS. E. GrigorievM. Yu. CheprasovYa. A. AhremenkoV. V. KolodzievaN. E. GoncharovL. A. KraevaSankt-Peterburg : NIIÈM imeni Pasteraarticleserratia, biofilms, extreme environment, psychrotolerant bacteria, cold adaptation, virulence, microbiological monitoringInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 3, Pp 585-590 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	serratia, biofilms, extreme environment, psychrotolerant bacteria, cold adaptation, virulence, microbiological monitoring Infectious and parasitic diseases RC109-216
spellingShingle	serratia, biofilms, extreme environment, psychrotolerant bacteria, cold adaptation, virulence, microbiological monitoring Infectious and parasitic diseases RC109-216 A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
description	The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains in this genus remains unexplored. The aim of this study was to compare the genomes of two Serratia strains isolated in polar regions, primarily examining genetic factors of virulence and adaptation to cryogenic environment. During the 56th Russian Antarctic Expedition the Serratia liquefaciens 72 strain was isolated from a guano sample of the Adelie Penguin (Pygoscelis adeliae) colony on Tokarev Island (Haswell Archipelago, East Antarctica). The Serratia fonticola 5l strain was isolated from the frozen carcass of moose (Alces alces) fossils found on the Buor-Khaya Peninsula near the Laptev Sea coast (Yakutia Region, Russia). The whole-genome sequencing of such strains allowed to reveal genetic structures evidencing about their successful adaptation to low temperatures. Thus, it was found that both genomes contain genes encoding the main cold shock proteins, phylogenetically close to the corresponding genes in the hypobarotolerant Serratia liquefaciens strain ATCC 27592. Furthermore, both strains bear a cluster of tc-fABCD genes determining the bacterial adhesion to epithelial tissues, and the genes for RTX toxins — adhesins, crucial factors of biofilm formation in pathogenic Gram-negative bacteria. Experimental studies confirmed the ability of Serratia liquefaciens 72 and Serratia fonticola 5l to actively form biofilms in a wide temperature range (from 6°C to 37°C). The results obtained indicate that the examined genus Serratia strains isolated in Arctica and Antarctica exert overall similar adaptation strategies to polar climate, including the ability to produce pili, show active adhesion, and biofilm formation under low temperatures. Genetic adaptive factors may also act as pathogenicity factors allowing extremotolerant Serratia strains to exert traits of opportunistic and nosocomial pathogens and spread via chilled food-borne transmission. The wide use of food technologies, such as cooling and vacuum sealing, can potentially create a new ecological niche favourable for selection of psychrotolerant and hypobarotolerant pathogens. The data obtained allow to raise a question about necessity of further studies to monitor genetic diversity among psychrophilic hypobarotolerant microbial populations possessing pathogenic and epidemic potential.
format	article
author	A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva
author_facet	A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva
author_sort	A. E. Goncharov
title	Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
title_short	Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
title_full	Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
title_fullStr	Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
title_full_unstemmed	Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats
title_sort	assessing <i>serratia</i> spp. pathogenic potential from cryogenic habitats
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6
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Assessing <i>Serratia</i> spp. pathogenic potential from cryogenic habitats

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