Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

Michela Morano,1,* Sandra Wrobel,2,3,* Federica Fregnan,1 Ofra Ziv-Polat,4 Abraham Shahar,4 Andreas Ratzka,2 Claudia Grothe,2,3 Stefano Geuna,1 Kirsten Haastert-Talini2,3 1Department of Clinical and Biological Sciences, Università Degli Studi di Torino, Orbassano, Piemonte, Italy; 2Insti...

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Autores principales: Morano M, Wrobel S, Fregnan F, Ziv-Polat O, Shahar A, Ratzka A, Grothe C, Geuna S, Haastert-Talini K
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Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
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spelling oai:doaj.org-article:4bcce0e9a4e34493874ad52d4d6296122021-12-02T02:41:15ZNanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials1178-2013https://doaj.org/article/4bcce0e9a4e34493874ad52d4d6296122014-11-01T00:00:00Zhttp://www.dovepress.com/nanotechnology-versus-stem-cell-engineering-innbspvitro-comparison-of--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Michela Morano,1,* Sandra Wrobel,2,3,* Federica Fregnan,1 Ofra Ziv-Polat,4 Abraham Shahar,4 Andreas Ratzka,2 Claudia Grothe,2,3 Stefano Geuna,1 Kirsten Haastert-Talini2,3 1Department of Clinical and Biological Sciences, Università Degli Studi di Torino, Orbassano, Piemonte, Italy; 2Institute of Neuroanatomy, Hannover Medical School, Hannover, Lower-Saxony, Germany; 3Center for Systems Neuroscience (ZSN), Hannover, Lower-Saxony, Germany; 4NVR Research Ltd, Ness-Ziona, Israel *These authors contributed equally to this work and share first authorship Purpose: Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits via nanotechnology or stem cell engineering and transplantation.Methods: Here, we comparatively investigated the bioactivity of selected neurotrophic factors conjugated to iron oxide nanoparticles (np-NTFs) and of bone marrow-derived stem cells genetically engineered to overexpress those neurotrophic factors (NTF-BMSCs). The neurite outgrowth inductive activity was monitored in culture systems of adult and neonatal rat sensory dorsal root ganglion neurons as well as in the cell line from rat pheochromocytoma (PC-12) cell sympathetic culture model system.Results: We demonstrate that np-NTFs reliably support numeric neurite outgrowth in all utilized culture models. In some aspects, especially with regard to their long-term bioactivity, np-NTFs are even superior to free NTFs. Engineered NTF-BMSCs proved to be less effective in induction of sensory neurite outgrowth but demonstrated an increased bioactivity in the PC-12 cell culture system. In contrast, primary nontransfected BMSCs were as effective as np-NTFs in sensory neurite induction and demonstrated an impairment of neuronal differentiation in the PC-12 cell system.Conclusion: Our results evidence that nanotechnology as used in our setup is superior over stem cell engineering when it comes to in vitro models for PNR. Furthermore, np-NTFs can easily be suspended in regenerative hydrogel matrix and could be delivered that way to nerve conduits for future in vivo studies and medical application. Keywords: iron oxide nanoparticles, conjugated neurotrophic factors, bone marrow-derived mesenchymal stem cells, genetic cell engineering, neurite outgrowthMorano MWrobel SFregnan FZiv-Polat OShahar ARatzka AGrothe CGeuna SHaastert-Talini KDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5289-5306 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Morano M
Wrobel S
Fregnan F
Ziv-Polat O
Shahar A
Ratzka A
Grothe C
Geuna S
Haastert-Talini K
Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
description Michela Morano,1,* Sandra Wrobel,2,3,* Federica Fregnan,1 Ofra Ziv-Polat,4 Abraham Shahar,4 Andreas Ratzka,2 Claudia Grothe,2,3 Stefano Geuna,1 Kirsten Haastert-Talini2,3 1Department of Clinical and Biological Sciences, Università Degli Studi di Torino, Orbassano, Piemonte, Italy; 2Institute of Neuroanatomy, Hannover Medical School, Hannover, Lower-Saxony, Germany; 3Center for Systems Neuroscience (ZSN), Hannover, Lower-Saxony, Germany; 4NVR Research Ltd, Ness-Ziona, Israel *These authors contributed equally to this work and share first authorship Purpose: Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits via nanotechnology or stem cell engineering and transplantation.Methods: Here, we comparatively investigated the bioactivity of selected neurotrophic factors conjugated to iron oxide nanoparticles (np-NTFs) and of bone marrow-derived stem cells genetically engineered to overexpress those neurotrophic factors (NTF-BMSCs). The neurite outgrowth inductive activity was monitored in culture systems of adult and neonatal rat sensory dorsal root ganglion neurons as well as in the cell line from rat pheochromocytoma (PC-12) cell sympathetic culture model system.Results: We demonstrate that np-NTFs reliably support numeric neurite outgrowth in all utilized culture models. In some aspects, especially with regard to their long-term bioactivity, np-NTFs are even superior to free NTFs. Engineered NTF-BMSCs proved to be less effective in induction of sensory neurite outgrowth but demonstrated an increased bioactivity in the PC-12 cell culture system. In contrast, primary nontransfected BMSCs were as effective as np-NTFs in sensory neurite induction and demonstrated an impairment of neuronal differentiation in the PC-12 cell system.Conclusion: Our results evidence that nanotechnology as used in our setup is superior over stem cell engineering when it comes to in vitro models for PNR. Furthermore, np-NTFs can easily be suspended in regenerative hydrogel matrix and could be delivered that way to nerve conduits for future in vivo studies and medical application. Keywords: iron oxide nanoparticles, conjugated neurotrophic factors, bone marrow-derived mesenchymal stem cells, genetic cell engineering, neurite outgrowth
format article
author Morano M
Wrobel S
Fregnan F
Ziv-Polat O
Shahar A
Ratzka A
Grothe C
Geuna S
Haastert-Talini K
author_facet Morano M
Wrobel S
Fregnan F
Ziv-Polat O
Shahar A
Ratzka A
Grothe C
Geuna S
Haastert-Talini K
author_sort Morano M
title Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
title_short Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
title_full Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
title_fullStr Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
title_full_unstemmed Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
title_sort nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/4bcce0e9a4e34493874ad52d4d629612
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