Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato

Secondary salinization caused by the overaccumulation of calcium nitrate [Ca(NO3)2] in soils due to excessive fertilization has become one of the major handicaps of protected vegetable production. Brassinolide, a bioactive plant steroid hormone, plays an important role in improving abiotic stress to...

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Autores principales:	Yi Zhang, Haoting Chen, Shuo Li, Yang Li, Mukesh Kumar Kanwar, Bin Li, Longqiang Bai, Jin Xu, Yu Shi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	calcium nitrate brassinolide stress responses antioxidative defence energy metabolism Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4bdfd9849e554e47b885ef49b6f46edd
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spelling	oai:doaj.org-article:4bdfd9849e554e47b885ef49b6f46edd2021-11-17T15:33:11ZComparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato1664-462X10.3389/fpls.2021.724288https://doaj.org/article/4bdfd9849e554e47b885ef49b6f46edd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.724288/fullhttps://doaj.org/toc/1664-462XSecondary salinization caused by the overaccumulation of calcium nitrate [Ca(NO3)2] in soils due to excessive fertilization has become one of the major handicaps of protected vegetable production. Brassinolide, a bioactive plant steroid hormone, plays an important role in improving abiotic stress tolerance in plants. However, whether and how brassinolide (BR) can alleviate Ca(NO3)2 stress remains elusive. Here, we investigated the effects of exogenous BR on hydroponically grown tomato (Solanum lycopersicum L.) plants under Ca(NO3)2 stress through proteomics combined with physiological studies. Proteomics analysis revealed that Ca(NO3)2 stress affected the accumulation of proteins involved in photosynthesis, stress responses, and antioxidant defense, however, exogenous BR increased the accumulation of proteins involved in chlorophyll metabolism and altered the osmotic stress responses in tomatoes under Ca(NO3)2 stress. Further physiological studies supported the results of proteomics and showed that the exogenous BR-induced alleviation of Ca(NO3)2 stress was associated with the improvement of photosynthetic efficiency, levels of soluble sugars and proteins, chlorophyll contents, and antioxidant enzyme activities, leading to the reduction in the levels of reactive oxygen species and membrane lipid peroxidation, and promotion of the recovery of photosynthetic performance, energy metabolism, and plant growth under Ca(NO3)2 stress. These results show the importance of applying BR in protected agriculture as a means for the effective management of secondary salinization.Yi ZhangHaoting ChenShuo LiYang LiMukesh Kumar KanwarBin LiLongqiang BaiJin XuYu ShiFrontiers Media S.A.articlecalcium nitratebrassinolidestress responsesantioxidative defenceenergy metabolismPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium nitrate brassinolide stress responses antioxidative defence energy metabolism Plant culture SB1-1110
spellingShingle	calcium nitrate brassinolide stress responses antioxidative defence energy metabolism Plant culture SB1-1110 Yi Zhang Haoting Chen Shuo Li Yang Li Mukesh Kumar Kanwar Bin Li Longqiang Bai Jin Xu Yu Shi Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
description	Secondary salinization caused by the overaccumulation of calcium nitrate [Ca(NO3)2] in soils due to excessive fertilization has become one of the major handicaps of protected vegetable production. Brassinolide, a bioactive plant steroid hormone, plays an important role in improving abiotic stress tolerance in plants. However, whether and how brassinolide (BR) can alleviate Ca(NO3)2 stress remains elusive. Here, we investigated the effects of exogenous BR on hydroponically grown tomato (Solanum lycopersicum L.) plants under Ca(NO3)2 stress through proteomics combined with physiological studies. Proteomics analysis revealed that Ca(NO3)2 stress affected the accumulation of proteins involved in photosynthesis, stress responses, and antioxidant defense, however, exogenous BR increased the accumulation of proteins involved in chlorophyll metabolism and altered the osmotic stress responses in tomatoes under Ca(NO3)2 stress. Further physiological studies supported the results of proteomics and showed that the exogenous BR-induced alleviation of Ca(NO3)2 stress was associated with the improvement of photosynthetic efficiency, levels of soluble sugars and proteins, chlorophyll contents, and antioxidant enzyme activities, leading to the reduction in the levels of reactive oxygen species and membrane lipid peroxidation, and promotion of the recovery of photosynthetic performance, energy metabolism, and plant growth under Ca(NO3)2 stress. These results show the importance of applying BR in protected agriculture as a means for the effective management of secondary salinization.
format	article
author	Yi Zhang Haoting Chen Shuo Li Yang Li Mukesh Kumar Kanwar Bin Li Longqiang Bai Jin Xu Yu Shi
author_facet	Yi Zhang Haoting Chen Shuo Li Yang Li Mukesh Kumar Kanwar Bin Li Longqiang Bai Jin Xu Yu Shi
author_sort	Yi Zhang
title	Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
title_short	Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
title_full	Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
title_fullStr	Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
title_full_unstemmed	Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato
title_sort	comparative physiological and proteomic analyses reveal the mechanisms of brassinolide-mediated tolerance to calcium nitrate stress in tomato
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4bdfd9849e554e47b885ef49b6f46edd
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Comparative Physiological and Proteomic Analyses Reveal the Mechanisms of Brassinolide-Mediated Tolerance to Calcium Nitrate Stress in Tomato

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