A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell....
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oai:doaj.org-article:4be05b799ca74e60ae224bf1cfb194ce2021-11-11T17:27:27ZA UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma10.3390/ijms2221120611422-00671661-6596https://doaj.org/article/4be05b799ca74e60ae224bf1cfb194ce2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12061https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell. Here, we examined a panel of normal human epidermal and nevus melanocytes and primary and metastatic melanoma cell lines to determine whether distinctive cell-intrinsic lipidomes can discern non-neoplastic from neoplastic melanocytes and define their metastatic potential. Lipidome profiles were obtained by UHPLC-ESI mass-spectrometry, and differences in the signatures were analyzed by multivariate statistical analyses. Significant and highly specific changes in more than 30 lipid species were annotated in the initiation of melanoma, whereas less numerous changes were associated with melanoma progression and the non-malignant transformation of nevus melanocytes. Notably, the “malignancy lipid signature” features marked drops in pivotal membrane lipids, like sphingomyelins, and aberrant elevation of ether-type lipids and phosphatidylglycerol and phosphatidylinositol variants, suggesting a previously undefined remodeling of sphingolipid and glycerophospholipid metabolism. Besides broadening the molecular definition of this neoplasm, the different lipid profiles identified may help improve the clinical diagnosis/prognosis and facilitate therapeutic interventions for cutaneous melanoma.Arantza Perez-ValleBeatriz Abad-GarcíaOlatz FresnedoGabriel Barreda-GómezPatricia AspichuetaAintzane AsumendiEgoitz AstigarragaJosé A. FernándezMaría Dolores BoyanoBegoña OchoaMDPI AGarticlelipid biomarkerlipid phenotypehumanmelanomamelanocytenevus melanocyteBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12061, p 12061 (2021) |
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| topic |
lipid biomarker lipid phenotype human melanoma melanocyte nevus melanocyte Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
lipid biomarker lipid phenotype human melanoma melanocyte nevus melanocyte Biology (General) QH301-705.5 Chemistry QD1-999 Arantza Perez-Valle Beatriz Abad-García Olatz Fresnedo Gabriel Barreda-Gómez Patricia Aspichueta Aintzane Asumendi Egoitz Astigarraga José A. Fernández María Dolores Boyano Begoña Ochoa A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| description |
Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell. Here, we examined a panel of normal human epidermal and nevus melanocytes and primary and metastatic melanoma cell lines to determine whether distinctive cell-intrinsic lipidomes can discern non-neoplastic from neoplastic melanocytes and define their metastatic potential. Lipidome profiles were obtained by UHPLC-ESI mass-spectrometry, and differences in the signatures were analyzed by multivariate statistical analyses. Significant and highly specific changes in more than 30 lipid species were annotated in the initiation of melanoma, whereas less numerous changes were associated with melanoma progression and the non-malignant transformation of nevus melanocytes. Notably, the “malignancy lipid signature” features marked drops in pivotal membrane lipids, like sphingomyelins, and aberrant elevation of ether-type lipids and phosphatidylglycerol and phosphatidylinositol variants, suggesting a previously undefined remodeling of sphingolipid and glycerophospholipid metabolism. Besides broadening the molecular definition of this neoplasm, the different lipid profiles identified may help improve the clinical diagnosis/prognosis and facilitate therapeutic interventions for cutaneous melanoma. |
| format |
article |
| author |
Arantza Perez-Valle Beatriz Abad-García Olatz Fresnedo Gabriel Barreda-Gómez Patricia Aspichueta Aintzane Asumendi Egoitz Astigarraga José A. Fernández María Dolores Boyano Begoña Ochoa |
| author_facet |
Arantza Perez-Valle Beatriz Abad-García Olatz Fresnedo Gabriel Barreda-Gómez Patricia Aspichueta Aintzane Asumendi Egoitz Astigarraga José A. Fernández María Dolores Boyano Begoña Ochoa |
| author_sort |
Arantza Perez-Valle |
| title |
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| title_short |
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| title_full |
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| title_fullStr |
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| title_full_unstemmed |
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma |
| title_sort |
uhplc-mass spectrometry view of human melanocytic cells uncovers potential lipid biomarkers of melanoma |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/4be05b799ca74e60ae224bf1cfb194ce |
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