Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro

The present study aims to study the effects of epidermal growth factors (EGF) supplementations to the in vitro maturation (IVM) medium of buffalo's oocytes on the oocyte maturation rate and embryo development competence. The ovaries were collected from El-Monieb slaughters house and transferred...

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Autores principales:	Nasra Ahmed Yousef, Hassan Abdel-Sabour Hussein, Magdy Ramadan Badr, Ahmed Ezzat Ahmed
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2018
Materias:	Bubalus bubalis Buffalo EGF IVM Oocytes Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4be5e8fe85904fd9bf1a98603d4f4fa1
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spelling	oai:doaj.org-article:4be5e8fe85904fd9bf1a98603d4f4fa12021-12-02T05:06:47Z Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro 2535-18262535-1877https://doaj.org/article/4be5e8fe85904fd9bf1a98603d4f4fa12018-11-01T00:00:00Zhttps://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/39/52https://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877The present study aims to study the effects of epidermal growth factors (EGF) supplementations to the in vitro maturation (IVM) medium of buffalo's oocytes on the oocyte maturation rate and embryo development competence. The ovaries were collected from El-Monieb slaughters house and transferred directly to the laboratory for the experiment processing. Excellent and good cumulus oocytes complexes (COCs) were aspirated from 3-8 mm diameter follicles. TCM-199 medium were used for in vitro maturation. In experiment I, COCs were incubated in EGF-containing IVM medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs at 38.5°C in humidified environment; 5% CO2 and 95% humidity. In experiment II, COCs were incubated in vitro fertilization (IVF) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. In experiment III, COCs were incubated in vitro culture (IVC) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. According to the expansion of oocytes and attaining the developmental embryo stages of morula and blastocyst, our results have shown that addition of 20 ng/ml of EGF to the IVM medium significantly increased the oocyte maturation and fertilization rates compared to those of CTL (76.96 ± 9.04 vs. 51.3 ± 4.66 and 67.96 ± 2.76 vs. 42.03 ± 4.83 %, respectively) (P<0.05). On the other hand, Addition of 20 ng/ml of EGF to the IVM medium significantly increased the fertilization rates compared to other concentrations (10 and 50 ng/ml) (67.96 ± 02.76 vs. 49.43 ± 03.67 and 34.70 ± 01.40, respectively). However, the developmental rates to morula and blastocyst stages in response to addition to IVC medium variably increased rather than those of control despite no significance. Nasra Ahmed YousefHassan Abdel-Sabour HusseinMagdy Ramadan BadrAhmed Ezzat Ahmed South Valley UniversityarticleBubalus bubalisBuffaloEGFIVMOocytesAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 1, Iss 2, Pp 85-94 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bubalus bubalis Buffalo EGF IVM Oocytes Agriculture S Veterinary medicine SF600-1100
spellingShingle	Bubalus bubalis Buffalo EGF IVM Oocytes Agriculture S Veterinary medicine SF600-1100 Nasra Ahmed Yousef Hassan Abdel-Sabour Hussein Magdy Ramadan Badr Ahmed Ezzat Ahmed Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
description	The present study aims to study the effects of epidermal growth factors (EGF) supplementations to the in vitro maturation (IVM) medium of buffalo's oocytes on the oocyte maturation rate and embryo development competence. The ovaries were collected from El-Monieb slaughters house and transferred directly to the laboratory for the experiment processing. Excellent and good cumulus oocytes complexes (COCs) were aspirated from 3-8 mm diameter follicles. TCM-199 medium were used for in vitro maturation. In experiment I, COCs were incubated in EGF-containing IVM medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs at 38.5°C in humidified environment; 5% CO2 and 95% humidity. In experiment II, COCs were incubated in vitro fertilization (IVF) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. In experiment III, COCs were incubated in vitro culture (IVC) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. According to the expansion of oocytes and attaining the developmental embryo stages of morula and blastocyst, our results have shown that addition of 20 ng/ml of EGF to the IVM medium significantly increased the oocyte maturation and fertilization rates compared to those of CTL (76.96 ± 9.04 vs. 51.3 ± 4.66 and 67.96 ± 2.76 vs. 42.03 ± 4.83 %, respectively) (P<0.05). On the other hand, Addition of 20 ng/ml of EGF to the IVM medium significantly increased the fertilization rates compared to other concentrations (10 and 50 ng/ml) (67.96 ± 02.76 vs. 49.43 ± 03.67 and 34.70 ± 01.40, respectively). However, the developmental rates to morula and blastocyst stages in response to addition to IVC medium variably increased rather than those of control despite no significance.
format	article
author	Nasra Ahmed Yousef Hassan Abdel-Sabour Hussein Magdy Ramadan Badr Ahmed Ezzat Ahmed
author_facet	Nasra Ahmed Yousef Hassan Abdel-Sabour Hussein Magdy Ramadan Badr Ahmed Ezzat Ahmed
author_sort	Nasra Ahmed Yousef
title	Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
title_short	Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
title_full	Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
title_fullStr	Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
title_full_unstemmed	Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro
title_sort	effect of epidermal growth factors (egf) on the maturation and developmental competence of buffalos’ oocytes and embryo stages in vitro
publisher	South Valley University
publishDate	2018
url	https://doaj.org/article/4be5e8fe85904fd9bf1a98603d4f4fa1
work_keys_str_mv	AT nasraahmedyousef effectofepidermalgrowthfactorsegfonthematurationanddevelopmentalcompetenceofbuffalosoocytesandembryostagesinvitro AT hassanabdelsabourhussein effectofepidermalgrowthfactorsegfonthematurationanddevelopmentalcompetenceofbuffalosoocytesandembryostagesinvitro AT magdyramadanbadr effectofepidermalgrowthfactorsegfonthematurationanddevelopmentalcompetenceofbuffalosoocytesandembryostagesinvitro AT ahmedezzatahmed effectofepidermalgrowthfactorsegfonthematurationanddevelopmentalcompetenceofbuffalosoocytesandembryostagesinvitro
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Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro

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