SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes.
Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and is counteracted by viral regulatory protein I...
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Public Library of Science (PLoS)
2011
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oai:doaj.org-article:4beb4b6c3fee4d3b9cba26b8e2ad8b3e2021-11-18T06:03:13ZSUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes.1553-73661553-737410.1371/journal.ppat.1002123https://doaj.org/article/4beb4b6c3fee4d3b9cba26b8e2ad8b3e2011-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779164/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and is counteracted by viral regulatory protein ICP0. We report that the SUMO interaction motifs of PML, Sp100 and hDaxx are required for recruitment of these repressive proteins to HSV-1 induced foci, which also contain SUMO conjugates and PIAS2β, a SUMO E3 ligase. SUMO modification of PML and elements of its tripartite motif (TRIM) are also required for recruitment in cells lacking endogenous PML. Mutants of PML isoform I and hDaxx that are not recruited to virus induced foci are unable to reproduce the repression of ICP0 null mutant HSV-1 infection mediated by their wild type counterparts. We conclude that recruitment of ND10 components to sites associated with HSV-1 genomes reflects a cellular defence against invading pathogen DNA that is regulated through the SUMO modification pathway.Delphine Cuchet-LourençoChris BoutellVera LukashchukKyle GrantAmanda SykesJill MurrayAnne OrrRoger D EverettPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 7, p e1002123 (2011) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
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Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Delphine Cuchet-Lourenço Chris Boutell Vera Lukashchuk Kyle Grant Amanda Sykes Jill Murray Anne Orr Roger D Everett SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| description |
Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and is counteracted by viral regulatory protein ICP0. We report that the SUMO interaction motifs of PML, Sp100 and hDaxx are required for recruitment of these repressive proteins to HSV-1 induced foci, which also contain SUMO conjugates and PIAS2β, a SUMO E3 ligase. SUMO modification of PML and elements of its tripartite motif (TRIM) are also required for recruitment in cells lacking endogenous PML. Mutants of PML isoform I and hDaxx that are not recruited to virus induced foci are unable to reproduce the repression of ICP0 null mutant HSV-1 infection mediated by their wild type counterparts. We conclude that recruitment of ND10 components to sites associated with HSV-1 genomes reflects a cellular defence against invading pathogen DNA that is regulated through the SUMO modification pathway. |
| format |
article |
| author |
Delphine Cuchet-Lourenço Chris Boutell Vera Lukashchuk Kyle Grant Amanda Sykes Jill Murray Anne Orr Roger D Everett |
| author_facet |
Delphine Cuchet-Lourenço Chris Boutell Vera Lukashchuk Kyle Grant Amanda Sykes Jill Murray Anne Orr Roger D Everett |
| author_sort |
Delphine Cuchet-Lourenço |
| title |
SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| title_short |
SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| title_full |
SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| title_fullStr |
SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| title_full_unstemmed |
SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| title_sort |
sumo pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/4beb4b6c3fee4d3b9cba26b8e2ad8b3e |
| work_keys_str_mv |
AT delphinecuchetlourenco sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT chrisboutell sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT veralukashchuk sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT kylegrant sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT amandasykes sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT jillmurray sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT anneorr sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes AT rogerdeverett sumopathwaydependentrecruitmentofcellularrepressorstoherpessimplexvirustype1genomes |
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1718424663065886720 |