Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study

Introduction: Heart failure (HF) secondary to acute myocardial infarction (AMI) is still a worldwide problem with a high mortality rate. The current study aimed to explore early and reliable predictive biomarkers of HF following AMI. Methods: The gene expression profile GSE59867 was downloaded from...

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Autores principales:	Congyi Yu, Wenjun Zhou
Formato:	article
Lenguaje:	EN PT
Publicado:	Elsevier 2021
Materias:	Enfarte do miocárdio Insuficiência cardíaca Neutrófilos Células CD4 T Bioinformática Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/4bf793b5757b43ba90436907e647241c
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id	oai:doaj.org-article:4bf793b5757b43ba90436907e647241c
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN PT
topic	Enfarte do miocárdio Insuficiência cardíaca Neutrófilos Células CD4 T Bioinformática Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	Enfarte do miocárdio Insuficiência cardíaca Neutrófilos Células CD4 T Bioinformática Diseases of the circulatory (Cardiovascular) system RC666-701 Congyi Yu Wenjun Zhou Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
description	Introduction: Heart failure (HF) secondary to acute myocardial infarction (AMI) is still a worldwide problem with a high mortality rate. The current study aimed to explore early and reliable predictive biomarkers of HF following AMI. Methods: The gene expression profile GSE59867 was downloaded from GEO. Array data from peripheral blood mononuclear cells (PBMCs) was used from 46 control patients and 111 patients with AMI at four time points: (i) first day of AMI; (ii) 4-6 days after AMI; (iii) one month after AMI; and (iv) six months after AMI. Among the 111 AMI patients, nine with HF and eight without HF were studied. CIBERSORT was used to analyze the relative proportions of immune cells in PBMCs. The proportions of immune cells in different groups were compared. Differentially expressed genes (DEGs) were analyzed with R language packages. Results: The percentages of monocytes and neutrophils increased significantly on the first day of AMI, and then decreased gradually. The percentage of regulatory T cells increased significantly 4-6 days after AMI, while the percentage of resting memory CD4 cells, CD8 T cells, and resting natural killer cells decreased significantly on the first day of AMI, and then increased gradually. Patients who developed HF had a significantly higher proportion of neutrophils in PBMCs on the first day of AMI, but had a significantly lower proportion of naive CD4 T cells. Two shared genes, interleukin-1 receptor 2 (IL1R2) and leucine-rich repeat neuronal protein 3 (LRRN3), were found to have potentially important roles in predicting the development of HF following AMI. Conclusion: A higher proportion of neutrophils and a lower proportion of naive CD4 T cells in PBMCs on the first day of AMI may be correlated with the development of HF following AMI. IL1R2 and LRRN3 may exert functions in the development of HF following AMI. Resumo: Objetivos: A insuficiência cardíaca (IC) secundária ao enfarte agudo do miocárdio (EAM) constitui um problema mundial devido à elevada taxa de mortalidade. Este estudo tem o objetivo de identificar biomarcadores preditores de IC pós o EAM. Métodos: O perfil da expressão do gene GSE59867 foi descarregado do GEO. Foram utilizados os dados da matriz das células sanguíneas mononucleares periféricas (CSMP), incluindo 46 doentes controlo e 111 doentes com EAM em quatro momentos: i) 1.° dia após EAM; II) 4–6 dias após EAM; iii) um mês após EAM; e iv) seis meses após EAM. Dos 111 doentes com EAM, foram estudados nove doentes com insuficiência cardíaca (IC) e oito sem IC. O CIBERSORT foi utilizado para analisar as proporções relativas das células imunes nas CSMP. As proporções das células imunes foram comparadas nos diferentes grupos. Os genes expressos de forma diferente (GEDs) foram analisados com pacotes de linguagem R. Resultados: A percentagem de monócitos e de neutrófilos aumentou significativamente no primeiro dia após o EAM e foi diminuindo gradualmente. A percentagem de Treg aumentou significativamente 4–6 dias após o EAM. A percentagem de repouso da memória CD4, as células CD8 T e as células de repouso NK diminuíram significativamente no primeiro dia após o EAM e seguidamente aumentaram gradualmente. Os doentes com prognóstico de IC apresentaram uma proporção mais elevada de neutrófilos nas CSMP no primeiro dia após o EAM, tendo, no entanto, apresentado uma proporção significativa menos elevada de células CD4 T. Dois genes partilhados, a interleucina 1 recetor 2 (IL1R2) e a proteína neuronal de repetição rica em leucina 3 (LRRN3), foram consideradas como tendo um papel potencialmente importante no prognóstico de IC após o EAM. Conclusão: Uma proporção mais elevada de neutrófilos e uma proporção mais baixa de células CD4 T naïve nas CSMP no primeiro dia após o EAM podem correlacionar-se com o prognóstico de EAM. A IL1R2 e a LRRN3) podem exercer funções potenciais no desenvolvimento da IC pós-EAM.
format	article
author	Congyi Yu Wenjun Zhou
author_facet	Congyi Yu Wenjun Zhou
author_sort	Congyi Yu
title	Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
title_short	Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
title_full	Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
title_fullStr	Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
title_full_unstemmed	Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study
title_sort	peripheral neutrophils and naive cd4 t cells predict the development of heart failure following acute myocardial infarction: a bioinformatic study
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4bf793b5757b43ba90436907e647241c
work_keys_str_mv	AT congyiyu peripheralneutrophilsandnaivecd4tcellspredictthedevelopmentofheartfailurefollowingacutemyocardialinfarctionabioinformaticstudy AT wenjunzhou peripheralneutrophilsandnaivecd4tcellspredictthedevelopmentofheartfailurefollowingacutemyocardialinfarctionabioinformaticstudy
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spelling	oai:doaj.org-article:4bf793b5757b43ba90436907e647241c2021-11-14T04:30:38ZPeripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study0870-255110.1016/j.repc.2020.12.011https://doaj.org/article/4bf793b5757b43ba90436907e647241c2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0870255121003802https://doaj.org/toc/0870-2551Introduction: Heart failure (HF) secondary to acute myocardial infarction (AMI) is still a worldwide problem with a high mortality rate. The current study aimed to explore early and reliable predictive biomarkers of HF following AMI. Methods: The gene expression profile GSE59867 was downloaded from GEO. Array data from peripheral blood mononuclear cells (PBMCs) was used from 46 control patients and 111 patients with AMI at four time points: (i) first day of AMI; (ii) 4-6 days after AMI; (iii) one month after AMI; and (iv) six months after AMI. Among the 111 AMI patients, nine with HF and eight without HF were studied. CIBERSORT was used to analyze the relative proportions of immune cells in PBMCs. The proportions of immune cells in different groups were compared. Differentially expressed genes (DEGs) were analyzed with R language packages. Results: The percentages of monocytes and neutrophils increased significantly on the first day of AMI, and then decreased gradually. The percentage of regulatory T cells increased significantly 4-6 days after AMI, while the percentage of resting memory CD4 cells, CD8 T cells, and resting natural killer cells decreased significantly on the first day of AMI, and then increased gradually. Patients who developed HF had a significantly higher proportion of neutrophils in PBMCs on the first day of AMI, but had a significantly lower proportion of naive CD4 T cells. Two shared genes, interleukin-1 receptor 2 (IL1R2) and leucine-rich repeat neuronal protein 3 (LRRN3), were found to have potentially important roles in predicting the development of HF following AMI. Conclusion: A higher proportion of neutrophils and a lower proportion of naive CD4 T cells in PBMCs on the first day of AMI may be correlated with the development of HF following AMI. IL1R2 and LRRN3 may exert functions in the development of HF following AMI. Resumo: Objetivos: A insuficiência cardíaca (IC) secundária ao enfarte agudo do miocárdio (EAM) constitui um problema mundial devido à elevada taxa de mortalidade. Este estudo tem o objetivo de identificar biomarcadores preditores de IC pós o EAM. Métodos: O perfil da expressão do gene GSE59867 foi descarregado do GEO. Foram utilizados os dados da matriz das células sanguíneas mononucleares periféricas (CSMP), incluindo 46 doentes controlo e 111 doentes com EAM em quatro momentos: i) 1.° dia após EAM; II) 4–6 dias após EAM; iii) um mês após EAM; e iv) seis meses após EAM. Dos 111 doentes com EAM, foram estudados nove doentes com insuficiência cardíaca (IC) e oito sem IC. O CIBERSORT foi utilizado para analisar as proporções relativas das células imunes nas CSMP. As proporções das células imunes foram comparadas nos diferentes grupos. Os genes expressos de forma diferente (GEDs) foram analisados com pacotes de linguagem R. Resultados: A percentagem de monócitos e de neutrófilos aumentou significativamente no primeiro dia após o EAM e foi diminuindo gradualmente. A percentagem de Treg aumentou significativamente 4–6 dias após o EAM. A percentagem de repouso da memória CD4, as células CD8 T e as células de repouso NK diminuíram significativamente no primeiro dia após o EAM e seguidamente aumentaram gradualmente. Os doentes com prognóstico de IC apresentaram uma proporção mais elevada de neutrófilos nas CSMP no primeiro dia após o EAM, tendo, no entanto, apresentado uma proporção significativa menos elevada de células CD4 T. Dois genes partilhados, a interleucina 1 recetor 2 (IL1R2) e a proteína neuronal de repetição rica em leucina 3 (LRRN3), foram consideradas como tendo um papel potencialmente importante no prognóstico de IC após o EAM. Conclusão: Uma proporção mais elevada de neutrófilos e uma proporção mais baixa de células CD4 T naïve nas CSMP no primeiro dia após o EAM podem correlacionar-se com o prognóstico de EAM. A IL1R2 e a LRRN3) podem exercer funções potenciais no desenvolvimento da IC pós-EAM.Congyi YuWenjun ZhouElsevierarticleEnfarte do miocárdioInsuficiência cardíacaNeutrófilosCélulas CD4 TBioinformáticaDiseases of the circulatory (Cardiovascular) systemRC666-701ENPTRevista Portuguesa de Cardiologia, Vol 40, Iss 11, Pp 839-847 (2021)

Peripheral neutrophils and naive CD4 T cells predict the development of heart failure following acute myocardial infarction: A bioinformatic study

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