Laser-ultrasonic delivery of agents into articular cartilage
Abstract Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induce...
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Nature Portfolio
2017
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oai:doaj.org-article:4c04c73ebf2b43d4b65dd64e478ad4142021-12-02T11:41:22ZLaser-ultrasonic delivery of agents into articular cartilage10.1038/s41598-017-04293-52045-2322https://doaj.org/article/4c04c73ebf2b43d4b65dd64e478ad4142017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04293-5https://doaj.org/toc/2045-2322Abstract Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induced plasma sound source) to deliver agents (methylene blue, MB, in PBS) into bovine AC. Treatment samples (n = 10) were immersed in MB solution simultaneously with LU exposure, while adjacent control 1 tissue (n = 10) was pre-treated with LU followed by immersion in MB and adjacent control 2 tissue (n = 10) was only immersed in MB. AC exposed (n = 22) or not exposed (n = 27) to LU were characterized for anomalies in structure, composition, viability or RNA expression. Optically detected MB content was significantly (p < 0.01) higher in treatment samples up to a depth of 500 µm from AC surface as compared to controls. No major unwanted short-term effects on AC structure, proteoglycan or collagen contents, chondrocyte viability or RNA expression levels were detected. In conclusion, LU can deliver agents into AC without major short-term concerns on safety. LU could reveal new strategies for the development of localized drug therapies in AC.Heikki J. NieminenGonçalo BarretoMikko A. FinniläAlejandro García-PérezAri SalmiSanjeev RanjanKari K. EklundKenneth P. H. PritzkerSimo SaarakkalaEdward HæggströmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Heikki J. Nieminen Gonçalo Barreto Mikko A. Finnilä Alejandro García-Pérez Ari Salmi Sanjeev Ranjan Kari K. Eklund Kenneth P. H. Pritzker Simo Saarakkala Edward Hæggström Laser-ultrasonic delivery of agents into articular cartilage |
| description |
Abstract Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induced plasma sound source) to deliver agents (methylene blue, MB, in PBS) into bovine AC. Treatment samples (n = 10) were immersed in MB solution simultaneously with LU exposure, while adjacent control 1 tissue (n = 10) was pre-treated with LU followed by immersion in MB and adjacent control 2 tissue (n = 10) was only immersed in MB. AC exposed (n = 22) or not exposed (n = 27) to LU were characterized for anomalies in structure, composition, viability or RNA expression. Optically detected MB content was significantly (p < 0.01) higher in treatment samples up to a depth of 500 µm from AC surface as compared to controls. No major unwanted short-term effects on AC structure, proteoglycan or collagen contents, chondrocyte viability or RNA expression levels were detected. In conclusion, LU can deliver agents into AC without major short-term concerns on safety. LU could reveal new strategies for the development of localized drug therapies in AC. |
| format |
article |
| author |
Heikki J. Nieminen Gonçalo Barreto Mikko A. Finnilä Alejandro García-Pérez Ari Salmi Sanjeev Ranjan Kari K. Eklund Kenneth P. H. Pritzker Simo Saarakkala Edward Hæggström |
| author_facet |
Heikki J. Nieminen Gonçalo Barreto Mikko A. Finnilä Alejandro García-Pérez Ari Salmi Sanjeev Ranjan Kari K. Eklund Kenneth P. H. Pritzker Simo Saarakkala Edward Hæggström |
| author_sort |
Heikki J. Nieminen |
| title |
Laser-ultrasonic delivery of agents into articular cartilage |
| title_short |
Laser-ultrasonic delivery of agents into articular cartilage |
| title_full |
Laser-ultrasonic delivery of agents into articular cartilage |
| title_fullStr |
Laser-ultrasonic delivery of agents into articular cartilage |
| title_full_unstemmed |
Laser-ultrasonic delivery of agents into articular cartilage |
| title_sort |
laser-ultrasonic delivery of agents into articular cartilage |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/4c04c73ebf2b43d4b65dd64e478ad414 |
| work_keys_str_mv |
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