Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink

Bioprinting offers great potential for the fabrication of three-dimensional living tissues by the precise layer-by-layer printing of biological materials, including living cells and cell-laden hydrogels. The laser-induced forward transfer (LIFT) of cell-laden bioinks is one of the most promising las...

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Autores principales:	Christina Kryou, Ioannis Theodorakos, Panagiotis Karakaidos, Apostolos Klinakis, Antonios Hatziapostolou, Ioanna Zergioti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laser-induced forward transfer time-resolved imaging laser fluence cell-laden bioink distance droplet/jet impingement Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/4c06f85749384b3bb2bb6fa4f74f24ca
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spelling	oai:doaj.org-article:4c06f85749384b3bb2bb6fa4f74f24ca2021-11-25T18:23:46ZParametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink10.3390/mi121114082072-666Xhttps://doaj.org/article/4c06f85749384b3bb2bb6fa4f74f24ca2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1408https://doaj.org/toc/2072-666XBioprinting offers great potential for the fabrication of three-dimensional living tissues by the precise layer-by-layer printing of biological materials, including living cells and cell-laden hydrogels. The laser-induced forward transfer (LIFT) of cell-laden bioinks is one of the most promising laser-printing technologies enabling biofabrication. However, for it to be a viable bioprinting technology, bioink printability must be carefully examined. In this study, we used a time-resolved imaging system to study the cell-laden bioink droplet formation process in terms of the droplet size, velocity, and traveling distance. For this purpose, the bioinks were prepared using breast cancer cells with different cell concentrations to evaluate the effect of the cell concentration on the droplet formation process and the survival of the cells after printing. These bioinks were compared with cell-free bioinks under the same printing conditions to understand the effect of the particle physical properties on the droplet formation procedure. The morphology of the printed droplets indicated that it is possible to print uniform droplets for a wide range of cell concentrations. Overall, it is concluded that the laser fluence and the distance of the donor–receiver substrates play an important role in the printing impingement type; consequently, a careful adjustment of these parameters can lead to high-quality printing.Christina KryouIoannis TheodorakosPanagiotis KarakaidosApostolos KlinakisAntonios HatziapostolouIoanna ZergiotiMDPI AGarticlelaser-induced forward transfertime-resolved imaginglaser fluencecell-laden bioinkdistancedroplet/jet impingementMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1408, p 1408 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laser-induced forward transfer time-resolved imaging laser fluence cell-laden bioink distance droplet/jet impingement Mechanical engineering and machinery TJ1-1570
spellingShingle	laser-induced forward transfer time-resolved imaging laser fluence cell-laden bioink distance droplet/jet impingement Mechanical engineering and machinery TJ1-1570 Christina Kryou Ioannis Theodorakos Panagiotis Karakaidos Apostolos Klinakis Antonios Hatziapostolou Ioanna Zergioti Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
description	Bioprinting offers great potential for the fabrication of three-dimensional living tissues by the precise layer-by-layer printing of biological materials, including living cells and cell-laden hydrogels. The laser-induced forward transfer (LIFT) of cell-laden bioinks is one of the most promising laser-printing technologies enabling biofabrication. However, for it to be a viable bioprinting technology, bioink printability must be carefully examined. In this study, we used a time-resolved imaging system to study the cell-laden bioink droplet formation process in terms of the droplet size, velocity, and traveling distance. For this purpose, the bioinks were prepared using breast cancer cells with different cell concentrations to evaluate the effect of the cell concentration on the droplet formation process and the survival of the cells after printing. These bioinks were compared with cell-free bioinks under the same printing conditions to understand the effect of the particle physical properties on the droplet formation procedure. The morphology of the printed droplets indicated that it is possible to print uniform droplets for a wide range of cell concentrations. Overall, it is concluded that the laser fluence and the distance of the donor–receiver substrates play an important role in the printing impingement type; consequently, a careful adjustment of these parameters can lead to high-quality printing.
format	article
author	Christina Kryou Ioannis Theodorakos Panagiotis Karakaidos Apostolos Klinakis Antonios Hatziapostolou Ioanna Zergioti
author_facet	Christina Kryou Ioannis Theodorakos Panagiotis Karakaidos Apostolos Klinakis Antonios Hatziapostolou Ioanna Zergioti
author_sort	Christina Kryou
title	Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
title_short	Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
title_full	Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
title_fullStr	Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
title_full_unstemmed	Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink
title_sort	parametric study of jet/droplet formation process during lift printing of living cell-laden bioink
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c06f85749384b3bb2bb6fa4f74f24ca
work_keys_str_mv	AT christinakryou parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink AT ioannistheodorakos parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink AT panagiotiskarakaidos parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink AT apostolosklinakis parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink AT antonioshatziapostolou parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink AT ioannazergioti parametricstudyofjetdropletformationprocessduringliftprintingoflivingcellladenbioink
_version_	1718411192665374720

Parametric Study of Jet/Droplet Formation Process during LIFT Printing of Living Cell-Laden Bioink

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