Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp

ABSTRACT In 2011, the human pneumococcal standard reference serum, 007sp, was established as a replacement for the previous standard, lot 89SF, supplies of which were dwindling. The pneumococcal reference serum is used primarily in the standardized pneumococcal enzyme-linked immunosorbent assay (Wor...

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Autores principales:	Scott Jones, Kier Finnegan, Jae Hee Wee, Polly D’Argoeuves, Lucy Roalfe, Suzanne Byrne, Sarah Heffernan, Adam Hunt, Marina Johnson, Tessa Jones, Emma Pearce, Hayley Richardson, Vasili Thalasselis, David Goldblatt
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	007sp ELISA Gram-positive bacteria IgA IgG1 IgG2 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4c11242829d74242897051cce23fc5a3
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spelling	oai:doaj.org-article:4c11242829d74242897051cce23fc5a32021-11-15T15:22:20ZAssignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp10.1128/mSphere.00400-192379-5042https://doaj.org/article/4c11242829d74242897051cce23fc5a32019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00400-19https://doaj.org/toc/2379-5042ABSTRACT In 2011, the human pneumococcal standard reference serum, 007sp, was established as a replacement for the previous standard, lot 89SF, supplies of which were dwindling. The pneumococcal reference serum is used primarily in the standardized pneumococcal enzyme-linked immunosorbent assay (World Health Organization reference enzyme-linked immunosorbent assay) but has also been used in functional assays. Serotype-specific IgG values for 24 pneumococcal capsular serotypes have previously been assigned to 007sp by bridging to the original values derived for lot 89SF. In this study, by bridging to existing values in lot 89SF, we assign weight-based serotype-specific IgA, IgG1, and IgG2 to 007sp for 11 pneumococcal capsular serotypes (1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F), as well as serotype 19A-specific IgA. Concentrations for serotype-specific IgA, IgG1, and IgG2 present in 007sp were comparable to those previously assigned to lot 89SF. In addition, the concentration of serotype-specific IgG1 plus IgG2 assigned to 007sp significantly correlated to previously assigned 007sp IgG values. The accuracy of antibody assignments to 007sp from lot 89SF was assessed by comparing the concentration of serotype-specific IgA, IgG1, and IgG2 in 16 unknown samples using both 007sp and lot 89SF as the standard. Interpolated values for the unknown samples were highly correlated with average R2 values of 0.9729, 0.9951, and 0.9933 for IgA, IgG1, and IgG2, respectively, for all serotypes demonstrating the precise nature assignments to 007sp made in this study. Nonparallelism between 007sp and lot 89SF has precluded the derivation of serotype-specific IgM values. IMPORTANCE A well-characterized antibody standard is an indispensable reagent for use in assays designed to measure antibodies with precision and where assays between laboratories need to be comparable. The human pneumococcal standard reference serum, lot 89SF, greatly facilitated the standardization of enzyme-linked immunosorbent assay methodologies during a critical period when the first pneumococcal polysaccharide-conjugate vaccines were being evaluated for licensure. Due to dwindling supplies of lot 89SF, a new reference standard, 007sp, was produced in 2011. Understanding the isotype and subclass composition of either natural or vaccine induced responses to pathogens has assumed increasing importance. In this study, we have assigned IgA, IgG1, and IgG2 values to pneumococcal serotypes 1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F by bridging to existing values in lot 89SF.Scott JonesKier FinneganJae Hee WeePolly D’ArgoeuvesLucy RoalfeSuzanne ByrneSarah HeffernanAdam HuntMarina JohnsonTessa JonesEmma PearceHayley RichardsonVasili ThalasselisDavid GoldblattAmerican Society for Microbiologyarticle007spELISAGram-positive bacteriaIgAIgG1IgG2MicrobiologyQR1-502ENmSphere, Vol 4, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	007sp ELISA Gram-positive bacteria IgA IgG1 IgG2 Microbiology QR1-502
spellingShingle	007sp ELISA Gram-positive bacteria IgA IgG1 IgG2 Microbiology QR1-502 Scott Jones Kier Finnegan Jae Hee Wee Polly D’Argoeuves Lucy Roalfe Suzanne Byrne Sarah Heffernan Adam Hunt Marina Johnson Tessa Jones Emma Pearce Hayley Richardson Vasili Thalasselis David Goldblatt Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
description	ABSTRACT In 2011, the human pneumococcal standard reference serum, 007sp, was established as a replacement for the previous standard, lot 89SF, supplies of which were dwindling. The pneumococcal reference serum is used primarily in the standardized pneumococcal enzyme-linked immunosorbent assay (World Health Organization reference enzyme-linked immunosorbent assay) but has also been used in functional assays. Serotype-specific IgG values for 24 pneumococcal capsular serotypes have previously been assigned to 007sp by bridging to the original values derived for lot 89SF. In this study, by bridging to existing values in lot 89SF, we assign weight-based serotype-specific IgA, IgG1, and IgG2 to 007sp for 11 pneumococcal capsular serotypes (1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F), as well as serotype 19A-specific IgA. Concentrations for serotype-specific IgA, IgG1, and IgG2 present in 007sp were comparable to those previously assigned to lot 89SF. In addition, the concentration of serotype-specific IgG1 plus IgG2 assigned to 007sp significantly correlated to previously assigned 007sp IgG values. The accuracy of antibody assignments to 007sp from lot 89SF was assessed by comparing the concentration of serotype-specific IgA, IgG1, and IgG2 in 16 unknown samples using both 007sp and lot 89SF as the standard. Interpolated values for the unknown samples were highly correlated with average R2 values of 0.9729, 0.9951, and 0.9933 for IgA, IgG1, and IgG2, respectively, for all serotypes demonstrating the precise nature assignments to 007sp made in this study. Nonparallelism between 007sp and lot 89SF has precluded the derivation of serotype-specific IgM values. IMPORTANCE A well-characterized antibody standard is an indispensable reagent for use in assays designed to measure antibodies with precision and where assays between laboratories need to be comparable. The human pneumococcal standard reference serum, lot 89SF, greatly facilitated the standardization of enzyme-linked immunosorbent assay methodologies during a critical period when the first pneumococcal polysaccharide-conjugate vaccines were being evaluated for licensure. Due to dwindling supplies of lot 89SF, a new reference standard, 007sp, was produced in 2011. Understanding the isotype and subclass composition of either natural or vaccine induced responses to pathogens has assumed increasing importance. In this study, we have assigned IgA, IgG1, and IgG2 values to pneumococcal serotypes 1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F by bridging to existing values in lot 89SF.
format	article
author	Scott Jones Kier Finnegan Jae Hee Wee Polly D’Argoeuves Lucy Roalfe Suzanne Byrne Sarah Heffernan Adam Hunt Marina Johnson Tessa Jones Emma Pearce Hayley Richardson Vasili Thalasselis David Goldblatt
author_facet	Scott Jones Kier Finnegan Jae Hee Wee Polly D’Argoeuves Lucy Roalfe Suzanne Byrne Sarah Heffernan Adam Hunt Marina Johnson Tessa Jones Emma Pearce Hayley Richardson Vasili Thalasselis David Goldblatt
author_sort	Scott Jones
title	Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
title_short	Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
title_full	Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
title_fullStr	Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
title_full_unstemmed	Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
title_sort	assignment of serotype-specific igg1, igg2, and iga weight-based antibody units to the human pneumococcal standard reference serum, 007sp
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/4c11242829d74242897051cce23fc5a3
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Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp

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