Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation

Abstract CD25, the alpha chain of the IL-2 receptor, is expressed on activated effector T cells that mediate immune graft damage. Induction immunosuppression is commonly used in solid organ transplantation and can include antibodies blocking CD25. However, regulatory T cells (Tregs) also rely on CD2...

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Autores principales:	Jacobo López-Abente, Marta Martínez-Bonet, Esther Bernaldo-de-Quirós, Manuela Camino, Nuria Gil, Esther Panadero, Juan Miguel Gil-Jaurena, Maribel Clemente, Simon Urschel, Lori West, Marjorie Pion, Rafael Correa-Rocha
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c11fa0c07a241ce8f6dbe296a31b180
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spelling	oai:doaj.org-article:4c11fa0c07a241ce8f6dbe296a31b1802021-12-02T14:12:09ZBasiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation10.1038/s41598-020-80567-92045-2322https://doaj.org/article/4c11fa0c07a241ce8f6dbe296a31b1802021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80567-9https://doaj.org/toc/2045-2322Abstract CD25, the alpha chain of the IL-2 receptor, is expressed on activated effector T cells that mediate immune graft damage. Induction immunosuppression is commonly used in solid organ transplantation and can include antibodies blocking CD25. However, regulatory T cells (Tregs) also rely on CD25 for their proliferation, survival, and regulatory function. Therefore, CD25-blockade may compromise Treg protective role against rejection. We analysed in vitro the effect of basiliximab (BXM) on the viability, phenotype, proliferation and cytokine production of Treg cells. We also evaluated in vivo the effect of BXM on Treg in thymectomized heart transplant children receiving BXM in comparison to patients not receiving induction therapy. Our results show that BXM reduces Treg counts and function in vitro by affecting their proliferation, Foxp3 expression, and IL-10 secretion capacity. In pediatric heart-transplant patients, we observed decreased Treg counts and a diminished Treg/Teff ratio in BXM-treated patients up to 6-month after treatment, recovering baseline values at the end of the 12-month follow up period. These results reveal that the use of BXM could produce detrimental effects on Tregs, and support the evidence suggesting that BXM induction could impair the protective role of Tregs in the period of highest incidence of acute graft rejection.Jacobo López-AbenteMarta Martínez-BonetEsther Bernaldo-de-QuirósManuela CaminoNuria GilEsther PanaderoJuan Miguel Gil-JaurenaMaribel ClementeSimon UrschelLori WestMarjorie PionRafael Correa-RochaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacobo López-Abente Marta Martínez-Bonet Esther Bernaldo-de-Quirós Manuela Camino Nuria Gil Esther Panadero Juan Miguel Gil-Jaurena Maribel Clemente Simon Urschel Lori West Marjorie Pion Rafael Correa-Rocha Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
description	Abstract CD25, the alpha chain of the IL-2 receptor, is expressed on activated effector T cells that mediate immune graft damage. Induction immunosuppression is commonly used in solid organ transplantation and can include antibodies blocking CD25. However, regulatory T cells (Tregs) also rely on CD25 for their proliferation, survival, and regulatory function. Therefore, CD25-blockade may compromise Treg protective role against rejection. We analysed in vitro the effect of basiliximab (BXM) on the viability, phenotype, proliferation and cytokine production of Treg cells. We also evaluated in vivo the effect of BXM on Treg in thymectomized heart transplant children receiving BXM in comparison to patients not receiving induction therapy. Our results show that BXM reduces Treg counts and function in vitro by affecting their proliferation, Foxp3 expression, and IL-10 secretion capacity. In pediatric heart-transplant patients, we observed decreased Treg counts and a diminished Treg/Teff ratio in BXM-treated patients up to 6-month after treatment, recovering baseline values at the end of the 12-month follow up period. These results reveal that the use of BXM could produce detrimental effects on Tregs, and support the evidence suggesting that BXM induction could impair the protective role of Tregs in the period of highest incidence of acute graft rejection.
format	article
author	Jacobo López-Abente Marta Martínez-Bonet Esther Bernaldo-de-Quirós Manuela Camino Nuria Gil Esther Panadero Juan Miguel Gil-Jaurena Maribel Clemente Simon Urschel Lori West Marjorie Pion Rafael Correa-Rocha
author_facet	Jacobo López-Abente Marta Martínez-Bonet Esther Bernaldo-de-Quirós Manuela Camino Nuria Gil Esther Panadero Juan Miguel Gil-Jaurena Maribel Clemente Simon Urschel Lori West Marjorie Pion Rafael Correa-Rocha
author_sort	Jacobo López-Abente
title	Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
title_short	Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
title_full	Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
title_fullStr	Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
title_full_unstemmed	Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation
title_sort	basiliximab impairs regulatory t cell (treg) function and could affect the short-term graft acceptance in children with heart transplantation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c11fa0c07a241ce8f6dbe296a31b180
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Basiliximab impairs regulatory T cell (TREG) function and could affect the short-term graft acceptance in children with heart transplantation

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