PCNA Loaders and Unloaders—One Ring That Rules Them All

During each cell duplication, the entirety of the genomic DNA in every cell must be accurately and quickly copied. Given the short time available for the chore, the requirement of many proteins, and the daunting amount of DNA present, DNA replication poses a serious challenge to the cell. A high lev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Matan Arbel, Karan Choudhary, Ofri Tfilin, Martin Kupiec
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RFC Elg1 Ctf18 PCNA clamp loading and unloading ubiquitin Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4c16086b4fb4463fbb01b2a99133f4a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4c16086b4fb4463fbb01b2a99133f4a8
record_format	dspace
spelling	oai:doaj.org-article:4c16086b4fb4463fbb01b2a99133f4a82021-11-25T17:42:16ZPCNA Loaders and Unloaders—One Ring That Rules Them All10.3390/genes121118122073-4425https://doaj.org/article/4c16086b4fb4463fbb01b2a99133f4a82021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1812https://doaj.org/toc/2073-4425During each cell duplication, the entirety of the genomic DNA in every cell must be accurately and quickly copied. Given the short time available for the chore, the requirement of many proteins, and the daunting amount of DNA present, DNA replication poses a serious challenge to the cell. A high level of coordination between polymerases and other DNA and chromatin-interacting proteins is vital to complete this task. One of the most important proteins for maintaining such coordination is PCNA. PCNA is a multitasking protein that forms a homotrimeric ring that encircles the DNA. It serves as a processivity factor for DNA polymerases and acts as a landing platform for different proteins interacting with DNA and chromatin. Therefore, PCNA is a signaling hub that influences the rate and accuracy of DNA replication, regulates DNA damage repair, controls chromatin formation during the replication, and the proper segregation of the sister chromatids. With so many essential roles, PCNA recruitment and turnover on the chromatin is of utmost importance. Three different, conserved protein complexes are in charge of loading/unloading PCNA onto DNA. Replication factor C (RFC) is the canonical complex in charge of loading PCNA during the S-phase. The Ctf18 and Elg1 (ATAD5 in mammalian) proteins form complexes similar to RFC, with particular functions in the cell’s nucleus. Here we summarize our current knowledge about the roles of these important factors in yeast and mammals.Matan ArbelKaran ChoudharyOfri TfilinMartin KupiecMDPI AGarticleRFCElg1Ctf18PCNAclamp loading and unloadingubiquitinGeneticsQH426-470ENGenes, Vol 12, Iss 1812, p 1812 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RFC Elg1 Ctf18 PCNA clamp loading and unloading ubiquitin Genetics QH426-470
spellingShingle	RFC Elg1 Ctf18 PCNA clamp loading and unloading ubiquitin Genetics QH426-470 Matan Arbel Karan Choudhary Ofri Tfilin Martin Kupiec PCNA Loaders and Unloaders—One Ring That Rules Them All
description	During each cell duplication, the entirety of the genomic DNA in every cell must be accurately and quickly copied. Given the short time available for the chore, the requirement of many proteins, and the daunting amount of DNA present, DNA replication poses a serious challenge to the cell. A high level of coordination between polymerases and other DNA and chromatin-interacting proteins is vital to complete this task. One of the most important proteins for maintaining such coordination is PCNA. PCNA is a multitasking protein that forms a homotrimeric ring that encircles the DNA. It serves as a processivity factor for DNA polymerases and acts as a landing platform for different proteins interacting with DNA and chromatin. Therefore, PCNA is a signaling hub that influences the rate and accuracy of DNA replication, regulates DNA damage repair, controls chromatin formation during the replication, and the proper segregation of the sister chromatids. With so many essential roles, PCNA recruitment and turnover on the chromatin is of utmost importance. Three different, conserved protein complexes are in charge of loading/unloading PCNA onto DNA. Replication factor C (RFC) is the canonical complex in charge of loading PCNA during the S-phase. The Ctf18 and Elg1 (ATAD5 in mammalian) proteins form complexes similar to RFC, with particular functions in the cell’s nucleus. Here we summarize our current knowledge about the roles of these important factors in yeast and mammals.
format	article
author	Matan Arbel Karan Choudhary Ofri Tfilin Martin Kupiec
author_facet	Matan Arbel Karan Choudhary Ofri Tfilin Martin Kupiec
author_sort	Matan Arbel
title	PCNA Loaders and Unloaders—One Ring That Rules Them All
title_short	PCNA Loaders and Unloaders—One Ring That Rules Them All
title_full	PCNA Loaders and Unloaders—One Ring That Rules Them All
title_fullStr	PCNA Loaders and Unloaders—One Ring That Rules Them All
title_full_unstemmed	PCNA Loaders and Unloaders—One Ring That Rules Them All
title_sort	pcna loaders and unloaders—one ring that rules them all
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c16086b4fb4463fbb01b2a99133f4a8
work_keys_str_mv	AT matanarbel pcnaloadersandunloadersoneringthatrulesthemall AT karanchoudhary pcnaloadersandunloadersoneringthatrulesthemall AT ofritfilin pcnaloadersandunloadersoneringthatrulesthemall AT martinkupiec pcnaloadersandunloadersoneringthatrulesthemall
_version_	1718412101898207232

PCNA Loaders and Unloaders—One Ring That Rules Them All

Ejemplares similares