Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized...

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Autores principales:	Liliya Kotliarevski, Karthik Ananth Mani, Reut Amar Feldbaum, Noga Yaakov, Eduard Belausov, Einat Zelinger, Dana Ment, Guy Mechrez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	titania mineral oil Pickering emulsion fungi conidia single-cell encapsulation Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4c22de9332c544f6ae8dbdcf9a3c6ed3
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spelling	oai:doaj.org-article:4c22de9332c544f6ae8dbdcf9a3c6ed32021-12-01T12:03:28ZSingle-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield2296-264610.3389/fchem.2021.726874https://doaj.org/article/4c22de9332c544f6ae8dbdcf9a3c6ed32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.726874/fullhttps://doaj.org/toc/2296-2646This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO2-NH2 or titania-NH2) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.Liliya KotliarevskiLiliya KotliarevskiKarthik Ananth ManiKarthik Ananth ManiReut Amar FeldbaumNoga YaakovEduard BelausovEinat ZelingerDana MentGuy MechrezFrontiers Media S.A.articletitaniamineral oilPickering emulsionfungiconidiasingle-cell encapsulationChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	titania mineral oil Pickering emulsion fungi conidia single-cell encapsulation Chemistry QD1-999
spellingShingle	titania mineral oil Pickering emulsion fungi conidia single-cell encapsulation Chemistry QD1-999 Liliya Kotliarevski Liliya Kotliarevski Karthik Ananth Mani Karthik Ananth Mani Reut Amar Feldbaum Noga Yaakov Eduard Belausov Einat Zelinger Dana Ment Guy Mechrez Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
description	This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO2-NH2 or titania-NH2) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.
format	article
author	Liliya Kotliarevski Liliya Kotliarevski Karthik Ananth Mani Karthik Ananth Mani Reut Amar Feldbaum Noga Yaakov Eduard Belausov Einat Zelinger Dana Ment Guy Mechrez
author_facet	Liliya Kotliarevski Liliya Kotliarevski Karthik Ananth Mani Karthik Ananth Mani Reut Amar Feldbaum Noga Yaakov Eduard Belausov Einat Zelinger Dana Ment Guy Mechrez
author_sort	Liliya Kotliarevski
title	Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
title_short	Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
title_full	Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
title_fullStr	Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
title_full_unstemmed	Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield
title_sort	single-conidium encapsulation in oil-in-water pickering emulsions at high encapsulation yield
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4c22de9332c544f6ae8dbdcf9a3c6ed3
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Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

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