Lubricin as a novel nanostructured protein coating to reduce fibroblast density

George Ejiofor Aninwene II,1 Zifan Yang,2 Vishnu Ravi,3 Gregory D Jay,2,4 Thomas J Webster1,51Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 2School of Engineering, Brown University, Providence, RI, USA; 3Albany Medical College, Albany, N...

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Autores principales:	Aninwene II GE, Yang Z, Ravi V, Jay GD, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4c24949ee10f41088aa8f21bc5448325
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spelling	oai:doaj.org-article:4c24949ee10f41088aa8f21bc54483252021-12-02T01:50:19ZLubricin as a novel nanostructured protein coating to reduce fibroblast density1178-2013https://doaj.org/article/4c24949ee10f41088aa8f21bc54483252014-06-01T00:00:00Zhttp://www.dovepress.com/lubricin-as-a-novel-nanostructured-protein-coating-to-reduce-fibroblas-a17370https://doaj.org/toc/1178-2013 George Ejiofor Aninwene II,1 Zifan Yang,2 Vishnu Ravi,3 Gregory D Jay,2,4 Thomas J Webster1,51Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 2School of Engineering, Brown University, Providence, RI, USA; 3Albany Medical College, Albany, NY, USA; 4Department of Emergency Medicine, Brown University, School of Medicine, Providence, RI, USA; 5Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: Excessive fibroblast adhesion and proliferation on the surface of medical implants (such as catheters, endotracheal tubes, intraocular lenses, etc) can lead to major postsurgical complications. This study showed that when coated on tissue culture polystyrene, lubricin, a nanostructured mucinous glycoprotein found in the synovial fluid of joints, decreased fibroblast density for up to 2 days of culture compared to controls treated with phosphate buffered saline (PBS). When examining why, similar antifibroblast density results were found when coating tissue culture polystyrene with bovine submaxillary mucin (BSM), an even smaller protein closely related to the central subregion of lubricin. Additionally, results from this study demonstrated that in contrast to BSM or controls (PBS-coated and non-coated samples), lubricin was better at preserving the health of nonadherent or loosely adherent fibroblasts; fibroblasts that did not adhere or loosely adhered on the lubricin-coated tissue culture polystyrene adhered and proliferated well for up to an additional day when they were reseeded on uncoated tissue culture polystyrene. In summary, this study provides evidence for the promise of nanostructured lubricin (and to a lesser extent BSM) to inhibit fibroblast adhesion and growth when coated on medical devices; lubricin should be further explored for numerous medical device applications.Keywords: lubricin, antiadhesive, fibroblasts, mucinAninwene II GEYang ZRavi VJay GDWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3131-3135 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Aninwene II GE Yang Z Ravi V Jay GD Webster TJ Lubricin as a novel nanostructured protein coating to reduce fibroblast density
description	George Ejiofor Aninwene II,1 Zifan Yang,2 Vishnu Ravi,3 Gregory D Jay,2,4 Thomas J Webster1,51Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 2School of Engineering, Brown University, Providence, RI, USA; 3Albany Medical College, Albany, NY, USA; 4Department of Emergency Medicine, Brown University, School of Medicine, Providence, RI, USA; 5Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: Excessive fibroblast adhesion and proliferation on the surface of medical implants (such as catheters, endotracheal tubes, intraocular lenses, etc) can lead to major postsurgical complications. This study showed that when coated on tissue culture polystyrene, lubricin, a nanostructured mucinous glycoprotein found in the synovial fluid of joints, decreased fibroblast density for up to 2 days of culture compared to controls treated with phosphate buffered saline (PBS). When examining why, similar antifibroblast density results were found when coating tissue culture polystyrene with bovine submaxillary mucin (BSM), an even smaller protein closely related to the central subregion of lubricin. Additionally, results from this study demonstrated that in contrast to BSM or controls (PBS-coated and non-coated samples), lubricin was better at preserving the health of nonadherent or loosely adherent fibroblasts; fibroblasts that did not adhere or loosely adhered on the lubricin-coated tissue culture polystyrene adhered and proliferated well for up to an additional day when they were reseeded on uncoated tissue culture polystyrene. In summary, this study provides evidence for the promise of nanostructured lubricin (and to a lesser extent BSM) to inhibit fibroblast adhesion and growth when coated on medical devices; lubricin should be further explored for numerous medical device applications.Keywords: lubricin, antiadhesive, fibroblasts, mucin
format	article
author	Aninwene II GE Yang Z Ravi V Jay GD Webster TJ
author_facet	Aninwene II GE Yang Z Ravi V Jay GD Webster TJ
author_sort	Aninwene II GE
title	Lubricin as a novel nanostructured protein coating to reduce fibroblast density
title_short	Lubricin as a novel nanostructured protein coating to reduce fibroblast density
title_full	Lubricin as a novel nanostructured protein coating to reduce fibroblast density
title_fullStr	Lubricin as a novel nanostructured protein coating to reduce fibroblast density
title_full_unstemmed	Lubricin as a novel nanostructured protein coating to reduce fibroblast density
title_sort	lubricin as a novel nanostructured protein coating to reduce fibroblast density
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/4c24949ee10f41088aa8f21bc5448325
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Lubricin as a novel nanostructured protein coating to reduce fibroblast density

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