Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass

Microbial lipids have similar fatty acid composition to plant oils, and therefore, are considered as an alternative feedstock for biodiesel production. Oleaginous yeasts accumulate considerable amounts of lipids intracellularly during growth on low-cost renewable feedstocks such as lignocellulosic b...

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Autores principales:	Marina Grubišić, Katarina Mihajlovski, Ana Marija Gruičić, Sunčica Beluhan, Božidar Šantek, Mirela Ivančić Šantek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oleaginous microorganism corn cobs biodiesel cellulases simultaneous saccharification and fermentation separate hydrolysis and fermentation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4c369443d728481ba17df62499707339
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spelling	oai:doaj.org-article:4c369443d728481ba17df624997073392021-11-25T18:05:55ZStrategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass10.3390/jof71109342309-608Xhttps://doaj.org/article/4c369443d728481ba17df624997073392021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/934https://doaj.org/toc/2309-608XMicrobial lipids have similar fatty acid composition to plant oils, and therefore, are considered as an alternative feedstock for biodiesel production. Oleaginous yeasts accumulate considerable amounts of lipids intracellularly during growth on low-cost renewable feedstocks such as lignocellulosic biomass. In this study, we cultivated yeast <i>Trichosporon oleaginosus</i> on hydrolysate of alkaline pretreated corn cobs. Different process configurations were evaluated and compared, including separate hydrolysis and fermentation (SHF) with cellulase recycle and simultaneous saccharification and fermentation (SSF) in batch and fed-batch mode. At low enzyme loading, the highest lipid concentration of 26.74 g L<sup>−1</sup> was reached in fed-batch SSF fed with 2.5% (g g<sup>−1</sup>) substrate. Batch SHF was conducted for four rounds with recycling the cellulase adsorbed on unhydrolyzed lignocellulosic biomass. Thirty percent of cellulase saving was achieved for rounds 2–4 without compromising productivity and lipid yield. The addition of Tween 80 to lignocellulosic slurry improved the hydrolysis rate of structural carbohydrates in pretreated lignocellulosic biomass. Furthermore, supplementing the growth medium with Tween 80 improved lipid yield and productivity without affecting yeast growth. Oleaginous yeast <i>T. oleaginosus</i> is a promising strain for the sustainable and efficient production of lipids from renewable lignocellulosic feedstock.Marina GrubišićKatarina MihajlovskiAna Marija GruičićSunčica BeluhanBožidar ŠantekMirela Ivančić ŠantekMDPI AGarticleoleaginous microorganismcorn cobsbiodieselcellulasessimultaneous saccharification and fermentationseparate hydrolysis and fermentationBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 934, p 934 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oleaginous microorganism corn cobs biodiesel cellulases simultaneous saccharification and fermentation separate hydrolysis and fermentation Biology (General) QH301-705.5
spellingShingle	oleaginous microorganism corn cobs biodiesel cellulases simultaneous saccharification and fermentation separate hydrolysis and fermentation Biology (General) QH301-705.5 Marina Grubišić Katarina Mihajlovski Ana Marija Gruičić Sunčica Beluhan Božidar Šantek Mirela Ivančić Šantek Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
description	Microbial lipids have similar fatty acid composition to plant oils, and therefore, are considered as an alternative feedstock for biodiesel production. Oleaginous yeasts accumulate considerable amounts of lipids intracellularly during growth on low-cost renewable feedstocks such as lignocellulosic biomass. In this study, we cultivated yeast <i>Trichosporon oleaginosus</i> on hydrolysate of alkaline pretreated corn cobs. Different process configurations were evaluated and compared, including separate hydrolysis and fermentation (SHF) with cellulase recycle and simultaneous saccharification and fermentation (SSF) in batch and fed-batch mode. At low enzyme loading, the highest lipid concentration of 26.74 g L<sup>−1</sup> was reached in fed-batch SSF fed with 2.5% (g g<sup>−1</sup>) substrate. Batch SHF was conducted for four rounds with recycling the cellulase adsorbed on unhydrolyzed lignocellulosic biomass. Thirty percent of cellulase saving was achieved for rounds 2–4 without compromising productivity and lipid yield. The addition of Tween 80 to lignocellulosic slurry improved the hydrolysis rate of structural carbohydrates in pretreated lignocellulosic biomass. Furthermore, supplementing the growth medium with Tween 80 improved lipid yield and productivity without affecting yeast growth. Oleaginous yeast <i>T. oleaginosus</i> is a promising strain for the sustainable and efficient production of lipids from renewable lignocellulosic feedstock.
format	article
author	Marina Grubišić Katarina Mihajlovski Ana Marija Gruičić Sunčica Beluhan Božidar Šantek Mirela Ivančić Šantek
author_facet	Marina Grubišić Katarina Mihajlovski Ana Marija Gruičić Sunčica Beluhan Božidar Šantek Mirela Ivančić Šantek
author_sort	Marina Grubišić
title	Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
title_short	Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
title_full	Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
title_fullStr	Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
title_full_unstemmed	Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass
title_sort	strategies for improvement of lipid production by yeast <i>trichosporon oleaginosus</i> from lignocellulosic biomass
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c369443d728481ba17df62499707339
work_keys_str_mv	AT marinagrubisic strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass AT katarinamihajlovski strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass AT anamarijagruicic strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass AT suncicabeluhan strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass AT bozidarsantek strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass AT mirelaivancicsantek strategiesforimprovementoflipidproductionbyyeastitrichosporonoleaginosusifromlignocellulosicbiomass
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Strategies for Improvement of Lipid Production by Yeast <i>Trichosporon oleaginosus</i> from Lignocellulosic Biomass

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