Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemi...

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Autores principales:	Yanan Zhao, Chuan Tian, Kunpeng Wu, Xueliang Zhou, Kexing Feng, Zhaonan Li, Zijian Wang, Xinwei Han
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	airway stent polycaprolactone vancomycin tracheal stenosis antibacterial Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4c3cfa35617c4b318e5d88c7f2f71fd2
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spelling	oai:doaj.org-article:4c3cfa35617c4b318e5d88c7f2f71fd22021-11-18T09:26:45ZVancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis2296-418510.3389/fbioe.2021.760395https://doaj.org/article/4c3cfa35617c4b318e5d88c7f2f71fd22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.760395/fullhttps://doaj.org/toc/2296-4185Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemical and biological properties of PVNF-n were evaluated by a series of tests, such as FT-IR, XRD, SEM-EDS, and antibacterial assay. The PVNF-n samples displayed a typical network structure of fibers with random directions. VA was successfully introduced into the PCL nanofibers and could be sustained and released. More importantly, PVNF-5 showed relatively good antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae (SPn). Thus, PVNF-5 was covered onto the self-expandable metallic stent and then implanted into a New Zealand rabbit model to repair tracheal stenosis. Compared to a metallic stent, a commercial pellosil matrix–covered stent, and a PVNF-0–covered metallic stent, the PVNF-5–covered airway stent showed reduced granulation tissue thickness, collagen density, α-SMA, CD68, TNF-α, IL-1, and IL-6 expression. In conclusion, this work provides an anti-infection film–covered airway stent that in site restrains tracheal stenosis.Yanan ZhaoYanan ZhaoChuan TianKunpeng WuXueliang ZhouKexing FengZhaonan LiZijian WangZijian WangXinwei HanFrontiers Media S.A.articleairway stentpolycaprolactonevancomycintracheal stenosisantibacterialBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	airway stent polycaprolactone vancomycin tracheal stenosis antibacterial Biotechnology TP248.13-248.65
spellingShingle	airway stent polycaprolactone vancomycin tracheal stenosis antibacterial Biotechnology TP248.13-248.65 Yanan Zhao Yanan Zhao Chuan Tian Kunpeng Wu Xueliang Zhou Kexing Feng Zhaonan Li Zijian Wang Zijian Wang Xinwei Han Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
description	Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemical and biological properties of PVNF-n were evaluated by a series of tests, such as FT-IR, XRD, SEM-EDS, and antibacterial assay. The PVNF-n samples displayed a typical network structure of fibers with random directions. VA was successfully introduced into the PCL nanofibers and could be sustained and released. More importantly, PVNF-5 showed relatively good antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae (SPn). Thus, PVNF-5 was covered onto the self-expandable metallic stent and then implanted into a New Zealand rabbit model to repair tracheal stenosis. Compared to a metallic stent, a commercial pellosil matrix–covered stent, and a PVNF-0–covered metallic stent, the PVNF-5–covered airway stent showed reduced granulation tissue thickness, collagen density, α-SMA, CD68, TNF-α, IL-1, and IL-6 expression. In conclusion, this work provides an anti-infection film–covered airway stent that in site restrains tracheal stenosis.
format	article
author	Yanan Zhao Yanan Zhao Chuan Tian Kunpeng Wu Xueliang Zhou Kexing Feng Zhaonan Li Zijian Wang Zijian Wang Xinwei Han
author_facet	Yanan Zhao Yanan Zhao Chuan Tian Kunpeng Wu Xueliang Zhou Kexing Feng Zhaonan Li Zijian Wang Zijian Wang Xinwei Han
author_sort	Yanan Zhao
title	Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
title_short	Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
title_full	Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
title_fullStr	Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
title_full_unstemmed	Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis
title_sort	vancomycin-loaded polycaprolactone electrospinning nanofibers modulate the airway interfaces to restrain tracheal stenosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4c3cfa35617c4b318e5d88c7f2f71fd2
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Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

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