Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.

Histomonas meleagridis is the aetiological agent of histomonosis or "blackhead disease". Histomonosis is of special importance today, because there is no effective treatment to prevent its occurrence with considerable losses for the poultry industry. Despite its importance only a few molec...

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Autores principales:	Ivana Bilic, Barbara Jaskulska, Rozenn Souillard, Dieter Liebhart, Michael Hess
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c3f5860b13048b49429ebde50037cb3
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spelling	oai:doaj.org-article:4c3f5860b13048b49429ebde50037cb32021-11-18T08:26:43ZMulti-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.1932-620310.1371/journal.pone.0092438https://doaj.org/article/4c3f5860b13048b49429ebde50037cb32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24658534/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Histomonas meleagridis is the aetiological agent of histomonosis or "blackhead disease". Histomonosis is of special importance today, because there is no effective treatment to prevent its occurrence with considerable losses for the poultry industry. Despite its importance only a few molecular studies have yet been performed to investigate the degree of genetic diversity between different isolates of this parasite. In the present study a collection of well defined samples, previously shown positive for the DNA of H. meleagridis, was used to investigate genetic relatedness of the parasite. Samples originated from 25 turkey flocks collected in France between 2007 and 2010. Additionally, diagnostic samples, collected at our Clinic in Vienna, from different European countries and Azerbaijan, during 2010 to 2013 were included in the analyses. For the analysis three different genetic loci were analyzed: 18S rRNA, α-actinin1 and rpb1 genes. To amplify partial sequences of α-actinin1 and rpb1 genes, primers specifically targeting H. meleagridis were designed. Following PCR, the sequences of 18S rRNA, α-actinin1 and rpb1 loci were analyzed. Phylogenetic analyses demonstrated separation of H. meleagridis isolates in two different clusters. The majority of isolates grouped within the cluster 1 and originated from different European countries. The cluster 2 was rare and predominantly found in samples originating from France. Considering that the genetic variability of clusters can be seen as two distinct genetic types we propose the term genotype instead of cluster.Ivana BilicBarbara JaskulskaRozenn SouillardDieter LiebhartMichael HessPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92438 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ivana Bilic Barbara Jaskulska Rozenn Souillard Dieter Liebhart Michael Hess Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
description	Histomonas meleagridis is the aetiological agent of histomonosis or "blackhead disease". Histomonosis is of special importance today, because there is no effective treatment to prevent its occurrence with considerable losses for the poultry industry. Despite its importance only a few molecular studies have yet been performed to investigate the degree of genetic diversity between different isolates of this parasite. In the present study a collection of well defined samples, previously shown positive for the DNA of H. meleagridis, was used to investigate genetic relatedness of the parasite. Samples originated from 25 turkey flocks collected in France between 2007 and 2010. Additionally, diagnostic samples, collected at our Clinic in Vienna, from different European countries and Azerbaijan, during 2010 to 2013 were included in the analyses. For the analysis three different genetic loci were analyzed: 18S rRNA, α-actinin1 and rpb1 genes. To amplify partial sequences of α-actinin1 and rpb1 genes, primers specifically targeting H. meleagridis were designed. Following PCR, the sequences of 18S rRNA, α-actinin1 and rpb1 loci were analyzed. Phylogenetic analyses demonstrated separation of H. meleagridis isolates in two different clusters. The majority of isolates grouped within the cluster 1 and originated from different European countries. The cluster 2 was rare and predominantly found in samples originating from France. Considering that the genetic variability of clusters can be seen as two distinct genetic types we propose the term genotype instead of cluster.
format	article
author	Ivana Bilic Barbara Jaskulska Rozenn Souillard Dieter Liebhart Michael Hess
author_facet	Ivana Bilic Barbara Jaskulska Rozenn Souillard Dieter Liebhart Michael Hess
author_sort	Ivana Bilic
title	Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
title_short	Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
title_full	Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
title_fullStr	Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
title_full_unstemmed	Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.
title_sort	multi-locus typing of histomonas meleagridis isolates demonstrates the existence of two different genotypes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4c3f5860b13048b49429ebde50037cb3
work_keys_str_mv	AT ivanabilic multilocustypingofhistomonasmeleagridisisolatesdemonstratestheexistenceoftwodifferentgenotypes AT barbarajaskulska multilocustypingofhistomonasmeleagridisisolatesdemonstratestheexistenceoftwodifferentgenotypes AT rozennsouillard multilocustypingofhistomonasmeleagridisisolatesdemonstratestheexistenceoftwodifferentgenotypes AT dieterliebhart multilocustypingofhistomonasmeleagridisisolatesdemonstratestheexistenceoftwodifferentgenotypes AT michaelhess multilocustypingofhistomonasmeleagridisisolatesdemonstratestheexistenceoftwodifferentgenotypes
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Multi-locus typing of Histomonas meleagridis isolates demonstrates the existence of two different genotypes.

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