Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation

Abstract Pseudorabies virus (PRV) is a pathogen of swine resulting in devastating disease and economic losses worldwide. Resveratrol (Res) exhibits inhibitory activity against a wide range of viruses. Despite these important advances, the molecular mechanism(s) by which Res exerts its broad biologic...

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Autores principales: Xinghong Zhao, Qiankun Cui, Qiuting Fu, Xu Song, Renyong Jia, Yi Yang, Yuanfeng Zou, Lixia Li, Changliang He, Xiaoxia Liang, Lizi Yin, Juchun Lin, Gang Ye, Gang Shu, Ling Zhao, Fei Shi, Cheng Lv, Zhongqiong Yin
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:4c42600e629449bd8d7aa08425620bd02021-12-02T12:32:40ZAntiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation10.1038/s41598-017-09365-02045-2322https://doaj.org/article/4c42600e629449bd8d7aa08425620bd02017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09365-0https://doaj.org/toc/2045-2322Abstract Pseudorabies virus (PRV) is a pathogen of swine resulting in devastating disease and economic losses worldwide. Resveratrol (Res) exhibits inhibitory activity against a wide range of viruses. Despite these important advances, the molecular mechanism(s) by which Res exerts its broad biological effects have not yet been elucidated. In this paper, the antiviral activity of Res against PRV and its mechanism of action were investigated. The results showed that Res potently inhibited PRV replication in a dose-dependent manner, with a 50% inhibition concentration of 17.17 μM. The inhibition of virus multiplication in the presence of Res was not attributed to direct inactivation or inhibition of viral entry into the host cells but to the inhibition of viral multiplication in host cells. Further studies demonstrated that Res is a potent inhibitor of both NF-κB activation and NF-κB-dependent gene expression through its ability to inhibit IκB kinase activity, which is the key regulator in NF-κB activation. Thus, the inhibitory effect of Res on PRV-induced cell death and gene expression may be due to its ability to inhibit the degradation of IκB kinase. These results provided a new alternative control measure for PRV infection and new insights into the antiviral mechanism of Res.Xinghong ZhaoQiankun CuiQiuting FuXu SongRenyong JiaYi YangYuanfeng ZouLixia LiChangliang HeXiaoxia LiangLizi YinJuchun LinGang YeGang ShuLing ZhaoFei ShiCheng LvZhongqiong YinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xinghong Zhao
Qiankun Cui
Qiuting Fu
Xu Song
Renyong Jia
Yi Yang
Yuanfeng Zou
Lixia Li
Changliang He
Xiaoxia Liang
Lizi Yin
Juchun Lin
Gang Ye
Gang Shu
Ling Zhao
Fei Shi
Cheng Lv
Zhongqiong Yin
Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
description Abstract Pseudorabies virus (PRV) is a pathogen of swine resulting in devastating disease and economic losses worldwide. Resveratrol (Res) exhibits inhibitory activity against a wide range of viruses. Despite these important advances, the molecular mechanism(s) by which Res exerts its broad biological effects have not yet been elucidated. In this paper, the antiviral activity of Res against PRV and its mechanism of action were investigated. The results showed that Res potently inhibited PRV replication in a dose-dependent manner, with a 50% inhibition concentration of 17.17 μM. The inhibition of virus multiplication in the presence of Res was not attributed to direct inactivation or inhibition of viral entry into the host cells but to the inhibition of viral multiplication in host cells. Further studies demonstrated that Res is a potent inhibitor of both NF-κB activation and NF-κB-dependent gene expression through its ability to inhibit IκB kinase activity, which is the key regulator in NF-κB activation. Thus, the inhibitory effect of Res on PRV-induced cell death and gene expression may be due to its ability to inhibit the degradation of IκB kinase. These results provided a new alternative control measure for PRV infection and new insights into the antiviral mechanism of Res.
format article
author Xinghong Zhao
Qiankun Cui
Qiuting Fu
Xu Song
Renyong Jia
Yi Yang
Yuanfeng Zou
Lixia Li
Changliang He
Xiaoxia Liang
Lizi Yin
Juchun Lin
Gang Ye
Gang Shu
Ling Zhao
Fei Shi
Cheng Lv
Zhongqiong Yin
author_facet Xinghong Zhao
Qiankun Cui
Qiuting Fu
Xu Song
Renyong Jia
Yi Yang
Yuanfeng Zou
Lixia Li
Changliang He
Xiaoxia Liang
Lizi Yin
Juchun Lin
Gang Ye
Gang Shu
Ling Zhao
Fei Shi
Cheng Lv
Zhongqiong Yin
author_sort Xinghong Zhao
title Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
title_short Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
title_full Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
title_fullStr Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
title_full_unstemmed Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of IκB kinase activation
title_sort antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of iκb kinase activation
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/4c42600e629449bd8d7aa08425620bd0
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