Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis

Abstract The usefulness of local collagenase in therapeutic approaches to solid tumors has been tested recently. In this study, we evaluate the safety and efficacy of intraperitoneal collagenase associated or not to mitomycin for treatment of colorectal peritoneal metastases in an experimental rat m...

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Autores principales:	D. García-Olmo, P. Villarejo Campos, J. Barambio, S. Garcia Gomez-Heras, L. Vega-Clemente, S. Olmedillas-Lopez, H. Guadalajara, M. Garcia-Arranz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c4571ad4d8841f8bec9a3ff5ac4df1d
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spelling	oai:doaj.org-article:4c4571ad4d8841f8bec9a3ff5ac4df1d2021-12-02T15:22:59ZIntraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis10.1038/s41598-020-79721-02045-2322https://doaj.org/article/4c4571ad4d8841f8bec9a3ff5ac4df1d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79721-0https://doaj.org/toc/2045-2322Abstract The usefulness of local collagenase in therapeutic approaches to solid tumors has been tested recently. In this study, we evaluate the safety and efficacy of intraperitoneal collagenase associated or not to mitomycin for treatment of colorectal peritoneal metastases in an experimental rat model. Using a fixed-dose procedure, we found that a dose of collagenase of 37 IU/mL administered for 15 min with a hyperthermia pump at 37.5 °C, both in isolation or associated to sequential treatment with intraperitoneal mitomycin, led to a macroscopic decrease in tumor volume as evaluated by the modified peritoneal cancer index (mPCI). Concerning the safety of the procedure, the animals showed no physiological or behavioral disorders during 8 weeks of follow-up. Local treatment for peritoneal metastases of colorectal origin with intraperitoneal collagenase has proved safe and effective in an experimental murine model. Therefore, the stroma-first approach by enzymatic breakdown of collagen from the tumor's extracellular matrix provides a new therapeutic target for colorectal peritoneal metastases.D. García-OlmoP. Villarejo CamposJ. BarambioS. Garcia Gomez-HerasL. Vega-ClementeS. Olmedillas-LopezH. GuadalajaraM. Garcia-ArranzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D. García-Olmo P. Villarejo Campos J. Barambio S. Garcia Gomez-Heras L. Vega-Clemente S. Olmedillas-Lopez H. Guadalajara M. Garcia-Arranz Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
description	Abstract The usefulness of local collagenase in therapeutic approaches to solid tumors has been tested recently. In this study, we evaluate the safety and efficacy of intraperitoneal collagenase associated or not to mitomycin for treatment of colorectal peritoneal metastases in an experimental rat model. Using a fixed-dose procedure, we found that a dose of collagenase of 37 IU/mL administered for 15 min with a hyperthermia pump at 37.5 °C, both in isolation or associated to sequential treatment with intraperitoneal mitomycin, led to a macroscopic decrease in tumor volume as evaluated by the modified peritoneal cancer index (mPCI). Concerning the safety of the procedure, the animals showed no physiological or behavioral disorders during 8 weeks of follow-up. Local treatment for peritoneal metastases of colorectal origin with intraperitoneal collagenase has proved safe and effective in an experimental murine model. Therefore, the stroma-first approach by enzymatic breakdown of collagen from the tumor's extracellular matrix provides a new therapeutic target for colorectal peritoneal metastases.
format	article
author	D. García-Olmo P. Villarejo Campos J. Barambio S. Garcia Gomez-Heras L. Vega-Clemente S. Olmedillas-Lopez H. Guadalajara M. Garcia-Arranz
author_facet	D. García-Olmo P. Villarejo Campos J. Barambio S. Garcia Gomez-Heras L. Vega-Clemente S. Olmedillas-Lopez H. Guadalajara M. Garcia-Arranz
author_sort	D. García-Olmo
title	Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
title_short	Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
title_full	Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
title_fullStr	Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
title_full_unstemmed	Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
title_sort	intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c4571ad4d8841f8bec9a3ff5ac4df1d
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Intraperitoneal collagenase as a novel therapeutic approach in an experimental model of colorectal peritoneal carcinomatosis

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