HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements

High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is o...

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Autores principales:	Paola Rivaro, Davide Vivado, Pierpaolo Falco, Carmela Ianni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f
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spelling	oai:doaj.org-article:4c47ff8a1caa4b30bfab358be052389f2021-11-11T19:55:11ZHPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements10.3390/w132130302073-4441https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/21/3030https://doaj.org/toc/2073-4441High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH<sub>3</sub>CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface.Paola RivaroDavide VivadoPierpaolo FalcoCarmela IanniMDPI AGarticlepHmeta-cresol purpleHPLCocean acidificationRoss SeaHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3030, p 3030 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
description	High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH<sub>3</sub>CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface.
format	article
author	Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni
author_facet	Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni
author_sort	Paola Rivaro
title	HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
title_short	HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
title_full	HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
title_fullStr	HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
title_full_unstemmed	HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
title_sort	hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f
work_keys_str_mv	AT paolarivaro hplcdadpurificationandcharacterizationofmetacresolpurpleforspectrophotometricseawaterphmeasurements AT davidevivado hplcdadpurificationandcharacterizationofmetacresolpurpleforspectrophotometricseawaterphmeasurements AT pierpaolofalco hplcdadpurificationandcharacterizationofmetacresolpurpleforspectrophotometricseawaterphmeasurements AT carmelaianni hplcdadpurificationandcharacterizationofmetacresolpurpleforspectrophotometricseawaterphmeasurements
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HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements

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