Modular microfluidics enables kinetic insight from time-resolved cryo-EM

There is a growing interest in performing time-resolved cryo-EM studies. Here, the authors present a time-resolved sample preparation method for cryo-EM called trEM, which uses a microfluidic device to initiate the biochemical reaction by rapid mixing of the components and then spraying the sample o...

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Autores principales:	Märt-Erik Mäeots, Byungjin Lee, Andrea Nans, Seung-Geun Jeong, Mohammad M. N. Esfahani, Shan Ding, Daniel J. Smith, Chang-Soo Lee, Sung Sik Lee, Matthias Peter, Radoslav I. Enchev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4c520c72571f46d9942706a8ffffcd24
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spelling	oai:doaj.org-article:4c520c72571f46d9942706a8ffffcd242021-12-02T18:34:21ZModular microfluidics enables kinetic insight from time-resolved cryo-EM10.1038/s41467-020-17230-42041-1723https://doaj.org/article/4c520c72571f46d9942706a8ffffcd242020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17230-4https://doaj.org/toc/2041-1723There is a growing interest in performing time-resolved cryo-EM studies. Here, the authors present a time-resolved sample preparation method for cryo-EM called trEM, which uses a microfluidic device to initiate the biochemical reaction by rapid mixing of the components and then spraying the sample onto a cryo-EM grid to snap-freeze it in a blot-free, automated manner within several milliseconds.Märt-Erik MäeotsByungjin LeeAndrea NansSeung-Geun JeongMohammad M. N. EsfahaniShan DingDaniel J. SmithChang-Soo LeeSung Sik LeeMatthias PeterRadoslav I. EnchevNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Märt-Erik Mäeots Byungjin Lee Andrea Nans Seung-Geun Jeong Mohammad M. N. Esfahani Shan Ding Daniel J. Smith Chang-Soo Lee Sung Sik Lee Matthias Peter Radoslav I. Enchev Modular microfluidics enables kinetic insight from time-resolved cryo-EM
description	There is a growing interest in performing time-resolved cryo-EM studies. Here, the authors present a time-resolved sample preparation method for cryo-EM called trEM, which uses a microfluidic device to initiate the biochemical reaction by rapid mixing of the components and then spraying the sample onto a cryo-EM grid to snap-freeze it in a blot-free, automated manner within several milliseconds.
format	article
author	Märt-Erik Mäeots Byungjin Lee Andrea Nans Seung-Geun Jeong Mohammad M. N. Esfahani Shan Ding Daniel J. Smith Chang-Soo Lee Sung Sik Lee Matthias Peter Radoslav I. Enchev
author_facet	Märt-Erik Mäeots Byungjin Lee Andrea Nans Seung-Geun Jeong Mohammad M. N. Esfahani Shan Ding Daniel J. Smith Chang-Soo Lee Sung Sik Lee Matthias Peter Radoslav I. Enchev
author_sort	Märt-Erik Mäeots
title	Modular microfluidics enables kinetic insight from time-resolved cryo-EM
title_short	Modular microfluidics enables kinetic insight from time-resolved cryo-EM
title_full	Modular microfluidics enables kinetic insight from time-resolved cryo-EM
title_fullStr	Modular microfluidics enables kinetic insight from time-resolved cryo-EM
title_full_unstemmed	Modular microfluidics enables kinetic insight from time-resolved cryo-EM
title_sort	modular microfluidics enables kinetic insight from time-resolved cryo-em
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4c520c72571f46d9942706a8ffffcd24
work_keys_str_mv	AT marterikmaeots modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT byungjinlee modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT andreanans modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT seunggeunjeong modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT mohammadmnesfahani modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT shanding modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT danieljsmith modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT changsoolee modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT sungsiklee modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT matthiaspeter modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem AT radoslavienchev modularmicrofluidicsenableskineticinsightfromtimeresolvedcryoem
_version_	1718377858810773504

Modular microfluidics enables kinetic insight from time-resolved cryo-EM

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