Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling

Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural con...

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Autores principales:	Kibrom B. Abreha, Erik Alexandersson, Svante Resjö, Åsa Lankinen, Daniela Sueldo, Farnusch Kaschani, Markus Kaiser, Renier A. L. van der Hoorn, Fredrik Levander, Erik Andreasson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ABPP apoplast proteomics serine hydrolases β-glycosidases potato Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4c5521147f00474d8b9d88ab663b36e3
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spelling	oai:doaj.org-article:4c5521147f00474d8b9d88ab663b36e32021-11-11T17:26:00ZLeaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling10.3390/ijms2221120331422-00671661-6596https://doaj.org/article/4c5521147f00474d8b9d88ab663b36e32021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12033https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.Kibrom B. AbrehaErik AlexanderssonSvante ResjöÅsa LankinenDaniela SueldoFarnusch KaschaniMarkus KaiserRenier A. L. van der HoornFredrik LevanderErik AndreassonMDPI AGarticleABPPapoplastproteomicsserine hydrolasesβ-glycosidasespotatoBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12033, p 12033 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ABPP apoplast proteomics serine hydrolases β-glycosidases potato Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ABPP apoplast proteomics serine hydrolases β-glycosidases potato Biology (General) QH301-705.5 Chemistry QD1-999 Kibrom B. Abreha Erik Alexandersson Svante Resjö Åsa Lankinen Daniela Sueldo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Fredrik Levander Erik Andreasson Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
description	Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
format	article
author	Kibrom B. Abreha Erik Alexandersson Svante Resjö Åsa Lankinen Daniela Sueldo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Fredrik Levander Erik Andreasson
author_facet	Kibrom B. Abreha Erik Alexandersson Svante Resjö Åsa Lankinen Daniela Sueldo Farnusch Kaschani Markus Kaiser Renier A. L. van der Hoorn Fredrik Levander Erik Andreasson
author_sort	Kibrom B. Abreha
title	Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
title_short	Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
title_full	Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
title_fullStr	Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
title_full_unstemmed	Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
title_sort	leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profiling
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c5521147f00474d8b9d88ab663b36e3
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Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling

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