Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
Abstract In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are c...
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Nature Portfolio
2021
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oai:doaj.org-article:4c5bd77a700f4c5c80c1af47d2d302792021-12-02T12:14:56ZExpansion and characterization of epithelial stem cells with potential for cyclical hair regeneration10.1038/s41598-020-80624-32045-2322https://doaj.org/article/4c5bd77a700f4c5c80c1af47d2d302792021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80624-3https://doaj.org/toc/2045-2322Abstract In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin β5 (Itgβ5)-triple-positive (CD34+/CD49f+/Itgβ5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy.Makoto TakeoKyosuke AsakawaKoh-ei ToyoshimaMiho OgawaJingJing TongTarou IriéMasayuki YanagisawaAkio SatoTakashi TsujiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Makoto Takeo Kyosuke Asakawa Koh-ei Toyoshima Miho Ogawa JingJing Tong Tarou Irié Masayuki Yanagisawa Akio Sato Takashi Tsuji Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| description |
Abstract In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin β5 (Itgβ5)-triple-positive (CD34+/CD49f+/Itgβ5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy. |
| format |
article |
| author |
Makoto Takeo Kyosuke Asakawa Koh-ei Toyoshima Miho Ogawa JingJing Tong Tarou Irié Masayuki Yanagisawa Akio Sato Takashi Tsuji |
| author_facet |
Makoto Takeo Kyosuke Asakawa Koh-ei Toyoshima Miho Ogawa JingJing Tong Tarou Irié Masayuki Yanagisawa Akio Sato Takashi Tsuji |
| author_sort |
Makoto Takeo |
| title |
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| title_short |
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| title_full |
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| title_fullStr |
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| title_full_unstemmed |
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| title_sort |
expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/4c5bd77a700f4c5c80c1af47d2d30279 |
| work_keys_str_mv |
AT makototakeo expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT kyosukeasakawa expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT koheitoyoshima expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT mihoogawa expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT jingjingtong expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT tarouirie expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT masayukiyanagisawa expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT akiosato expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration AT takashitsuji expansionandcharacterizationofepithelialstemcellswithpotentialforcyclicalhairregeneration |
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1718394571825610752 |