Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between...
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2013
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oai:doaj.org-article:4c630a71e6bd497a9c7618da9923f0682021-11-18T08:57:59ZAflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.1932-620310.1371/journal.pone.0073004https://doaj.org/article/4c630a71e6bd497a9c7618da9923f0682013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015284/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between AFB1, miR-33a and β-catenin in human carcinoma cells. The level of miR-33a was up-regulated in hepatocellular carcinoma (HCC) cells treated with AFB1, while in the same cells causing the decrease in β-catenin expression when treated at their IC50 values. miR-33a, specifically miR-33a-5p, was demonstrated to down-regulate the expression of β-catenin, affect the β-catenin pathway, and inhibit cell growth. Also, by employing a luciferase assay, we found that miR-33a down-regulated β-catenin by directly binding to the 3'-UTR of β-catenin. These results suggested that AFB1 might down-regulate β-catenin by up-regulating miR-33a. This understanding opens new lines of thought in the potential role of miR-33a in the clinical therapy of cancer.Yi FangYoujun FengTongjin WuSwaminath SrinivasWeiqiang YangJue FanChi YangShihua WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73004 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| description |
MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between AFB1, miR-33a and β-catenin in human carcinoma cells. The level of miR-33a was up-regulated in hepatocellular carcinoma (HCC) cells treated with AFB1, while in the same cells causing the decrease in β-catenin expression when treated at their IC50 values. miR-33a, specifically miR-33a-5p, was demonstrated to down-regulate the expression of β-catenin, affect the β-catenin pathway, and inhibit cell growth. Also, by employing a luciferase assay, we found that miR-33a down-regulated β-catenin by directly binding to the 3'-UTR of β-catenin. These results suggested that AFB1 might down-regulate β-catenin by up-regulating miR-33a. This understanding opens new lines of thought in the potential role of miR-33a in the clinical therapy of cancer. |
| format |
article |
| author |
Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang |
| author_facet |
Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang |
| author_sort |
Yi Fang |
| title |
Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| title_short |
Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| title_full |
Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| title_fullStr |
Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| title_full_unstemmed |
Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a. |
| title_sort |
aflatoxin b1 negatively regulates wnt/β-catenin signaling pathway through activating mir-33a. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/4c630a71e6bd497a9c7618da9923f068 |
| work_keys_str_mv |
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