Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.

MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between...

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Auteurs principaux:	Yi Fang, Youjun Feng, Tongjin Wu, Swaminath Srinivas, Weiqiang Yang, Jue Fan, Chi Yang, Shihua Wang
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/4c630a71e6bd497a9c7618da9923f068
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spelling	oai:doaj.org-article:4c630a71e6bd497a9c7618da9923f0682021-11-18T08:57:59ZAflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.1932-620310.1371/journal.pone.0073004https://doaj.org/article/4c630a71e6bd497a9c7618da9923f0682013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015284/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between AFB1, miR-33a and β-catenin in human carcinoma cells. The level of miR-33a was up-regulated in hepatocellular carcinoma (HCC) cells treated with AFB1, while in the same cells causing the decrease in β-catenin expression when treated at their IC50 values. miR-33a, specifically miR-33a-5p, was demonstrated to down-regulate the expression of β-catenin, affect the β-catenin pathway, and inhibit cell growth. Also, by employing a luciferase assay, we found that miR-33a down-regulated β-catenin by directly binding to the 3'-UTR of β-catenin. These results suggested that AFB1 might down-regulate β-catenin by up-regulating miR-33a. This understanding opens new lines of thought in the potential role of miR-33a in the clinical therapy of cancer.Yi FangYoujun FengTongjin WuSwaminath SrinivasWeiqiang YangJue FanChi YangShihua WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73004 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
description	MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between AFB1, miR-33a and β-catenin in human carcinoma cells. The level of miR-33a was up-regulated in hepatocellular carcinoma (HCC) cells treated with AFB1, while in the same cells causing the decrease in β-catenin expression when treated at their IC50 values. miR-33a, specifically miR-33a-5p, was demonstrated to down-regulate the expression of β-catenin, affect the β-catenin pathway, and inhibit cell growth. Also, by employing a luciferase assay, we found that miR-33a down-regulated β-catenin by directly binding to the 3'-UTR of β-catenin. These results suggested that AFB1 might down-regulate β-catenin by up-regulating miR-33a. This understanding opens new lines of thought in the potential role of miR-33a in the clinical therapy of cancer.
format	article
author	Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang
author_facet	Yi Fang Youjun Feng Tongjin Wu Swaminath Srinivas Weiqiang Yang Jue Fan Chi Yang Shihua Wang
author_sort	Yi Fang
title	Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
title_short	Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
title_full	Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
title_fullStr	Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
title_full_unstemmed	Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.
title_sort	aflatoxin b1 negatively regulates wnt/β-catenin signaling pathway through activating mir-33a.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4c630a71e6bd497a9c7618da9923f068
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Aflatoxin B1 negatively regulates Wnt/β-catenin signaling pathway through activating miR-33a.

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