Grifola frondosa (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in C. elegans through the DAF-16/FOXO and SKN-1/NRF2 Signalling Pathways

In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharid...

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Autores principales:	Paula Aranaz, Adriana Peña, Ariane Vettorazzi, María José Fabra, Antonio Martínez-Abad, Amparo López-Rubio, Joan Pera, Javier Parladé, Massimo Castellari, Fermín I. Milagro, Carlos J. González-Navarro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bioactive compounds nutraceutical fungi obesity metabolic syndrome insulin food ingredients Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/4c65e559474345b980bee60a65cbea8c
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spelling	oai:doaj.org-article:4c65e559474345b980bee60a65cbea8c2021-11-25T18:35:41Z<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways10.3390/nu131139682072-6643https://doaj.org/article/4c65e559474345b980bee60a65cbea8c2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3968https://doaj.org/toc/2072-6643In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the <i>C. elegans</i> model to investigate the potential activities of a <i>Grifola frondosa</i> extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a <i>SOS/umu</i> test, significantly reduced the fat content of <i>C. elegans</i>, decreased the production of intracellular ROS and aging–lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the <i>daf-2/daf-16</i> and <i>skn-1/nrf-2</i> signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances.Paula AranazAdriana PeñaAriane VettorazziMaría José FabraAntonio Martínez-AbadAmparo López-RubioJoan PeraJavier ParladéMassimo CastellariFermín I. MilagroCarlos J. González-NavarroMDPI AGarticlebioactive compoundsnutraceutical fungiobesitymetabolic syndromeinsulinfood ingredientsNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3968, p 3968 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive compounds nutraceutical fungi obesity metabolic syndrome insulin food ingredients Nutrition. Foods and food supply TX341-641
spellingShingle	bioactive compounds nutraceutical fungi obesity metabolic syndrome insulin food ingredients Nutrition. Foods and food supply TX341-641 Paula Aranaz Adriana Peña Ariane Vettorazzi María José Fabra Antonio Martínez-Abad Amparo López-Rubio Joan Pera Javier Parladé Massimo Castellari Fermín I. Milagro Carlos J. González-Navarro <i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
description	In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the <i>C. elegans</i> model to investigate the potential activities of a <i>Grifola frondosa</i> extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a <i>SOS/umu</i> test, significantly reduced the fat content of <i>C. elegans</i>, decreased the production of intracellular ROS and aging–lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the <i>daf-2/daf-16</i> and <i>skn-1/nrf-2</i> signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances.
format	article
author	Paula Aranaz Adriana Peña Ariane Vettorazzi María José Fabra Antonio Martínez-Abad Amparo López-Rubio Joan Pera Javier Parladé Massimo Castellari Fermín I. Milagro Carlos J. González-Navarro
author_facet	Paula Aranaz Adriana Peña Ariane Vettorazzi María José Fabra Antonio Martínez-Abad Amparo López-Rubio Joan Pera Javier Parladé Massimo Castellari Fermín I. Milagro Carlos J. González-Navarro
author_sort	Paula Aranaz
title	<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
title_short	<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
title_full	<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
title_fullStr	<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
title_full_unstemmed	<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways
title_sort	<i>grifola frondosa</i> (maitake) extract reduces fat accumulation and improves health span in <i>c. elegans</i> through the <i>daf-16/foxo</i> and <i>skn-1/nrf2</i> signalling pathways
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c65e559474345b980bee60a65cbea8c
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<i>Grifola frondosa</i> (Maitake) Extract Reduces Fat Accumulation and Improves Health Span in <i>C. elegans</i> through the <i>DAF-16/FOXO</i> and <i>SKN-1/NRF2</i> Signalling Pathways

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