Antioxidant enzymes in canine mammary tumors

Spontaneous mammary tumors are very common in bitches. The involvement of oxidative stress and the function of antioxidant enzymes in cancerogenesis have been studied in depth in human medicine, while data in veterinary medicine are still fragmentary. The main aim of this study was to evaluate the a...

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Autores principales:	Andreani Giulia, Avallone Giancarlo, Ferlizza Enea, Isani Gloria
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	cancer dog superoxide dismutase catalase Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4c66f22a135d47209c92fabe67d0ac4f
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spelling	oai:doaj.org-article:4c66f22a135d47209c92fabe67d0ac4f2021-11-17T21:27:51ZAntioxidant enzymes in canine mammary tumors1820-744810.1515/acve-2017-0011https://doaj.org/article/4c66f22a135d47209c92fabe67d0ac4f2017-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0011https://doaj.org/toc/1820-7448Spontaneous mammary tumors are very common in bitches. The involvement of oxidative stress and the function of antioxidant enzymes in cancerogenesis have been studied in depth in human medicine, while data in veterinary medicine are still fragmentary. The main aim of this study was to evaluate the activity and the expression of superoxide dismutases (Cu-ZnSOD and MnSOD) and the activity of catalase (CAT) in canine mammary tumors in comparison with the adjacent healthy tissue. Six female dogs (mean age 10.4 years) were included in this study. After surgery, fresh tumor and healthy tissue samples were immediately frozen in dry ice and stored at −80°C for biochemical analyses, while the remaining parts were used for histopathological analysis. Enzyme activity was measured by spectrophotometric assays and protein expression by western blotting. In canine mammary tumors, Cu-ZnSOD activity and expression increased significantly compared with healthy control tissues (p=0.03). MnSOD showed a significantly lower activity in tumoral tissues at stage 2 (p<0.05), while a significant increase of expression was measured in tumors. CAT activity was significantly higher in healthy tissues respect to tumors (p=0.015). These variations of antioxidant enzymes activities and expression could be related to an increase of oxidative stress in breast cancer tissues and could be considered as biomarker candidates for neoplastic transformation.Andreani GiuliaAvallone GiancarloFerlizza EneaIsani GloriaSciendoarticlecancerdogsuperoxide dismutasecatalaseVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 1, Pp 121-130 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cancer dog superoxide dismutase catalase Veterinary medicine SF600-1100
spellingShingle	cancer dog superoxide dismutase catalase Veterinary medicine SF600-1100 Andreani Giulia Avallone Giancarlo Ferlizza Enea Isani Gloria Antioxidant enzymes in canine mammary tumors
description	Spontaneous mammary tumors are very common in bitches. The involvement of oxidative stress and the function of antioxidant enzymes in cancerogenesis have been studied in depth in human medicine, while data in veterinary medicine are still fragmentary. The main aim of this study was to evaluate the activity and the expression of superoxide dismutases (Cu-ZnSOD and MnSOD) and the activity of catalase (CAT) in canine mammary tumors in comparison with the adjacent healthy tissue. Six female dogs (mean age 10.4 years) were included in this study. After surgery, fresh tumor and healthy tissue samples were immediately frozen in dry ice and stored at −80°C for biochemical analyses, while the remaining parts were used for histopathological analysis. Enzyme activity was measured by spectrophotometric assays and protein expression by western blotting. In canine mammary tumors, Cu-ZnSOD activity and expression increased significantly compared with healthy control tissues (p=0.03). MnSOD showed a significantly lower activity in tumoral tissues at stage 2 (p<0.05), while a significant increase of expression was measured in tumors. CAT activity was significantly higher in healthy tissues respect to tumors (p=0.015). These variations of antioxidant enzymes activities and expression could be related to an increase of oxidative stress in breast cancer tissues and could be considered as biomarker candidates for neoplastic transformation.
format	article
author	Andreani Giulia Avallone Giancarlo Ferlizza Enea Isani Gloria
author_facet	Andreani Giulia Avallone Giancarlo Ferlizza Enea Isani Gloria
author_sort	Andreani Giulia
title	Antioxidant enzymes in canine mammary tumors
title_short	Antioxidant enzymes in canine mammary tumors
title_full	Antioxidant enzymes in canine mammary tumors
title_fullStr	Antioxidant enzymes in canine mammary tumors
title_full_unstemmed	Antioxidant enzymes in canine mammary tumors
title_sort	antioxidant enzymes in canine mammary tumors
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/4c66f22a135d47209c92fabe67d0ac4f
work_keys_str_mv	AT andreanigiulia antioxidantenzymesincaninemammarytumors AT avallonegiancarlo antioxidantenzymesincaninemammarytumors AT ferlizzaenea antioxidantenzymesincaninemammarytumors AT isanigloria antioxidantenzymesincaninemammarytumors
_version_	1718425354774773760

Antioxidant enzymes in canine mammary tumors

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