Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults

Abstract Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human...

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Autores principales:	Niccolò Terrando, John J. Park, Michael Devinney, Cliburn Chan, Mary Cooter, Pallavi Avasarala, Joseph P. Mathew, Quintin J. Quinones, Krishna Rao Maddipati, Miles Berger, for the MADCO-PC Study Team
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4c6cc38806d44862bca6b02bf708d86d
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spelling	oai:doaj.org-article:4c6cc38806d44862bca6b02bf708d86d2021-12-02T10:44:08ZImmunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults10.1038/s41598-021-82606-52045-2322https://doaj.org/article/4c6cc38806d44862bca6b02bf708d86d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82606-5https://doaj.org/toc/2045-2322Abstract Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography–mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.Niccolò TerrandoJohn J. ParkMichael DevinneyCliburn ChanMary CooterPallavi AvasaralaJoseph P. MathewQuintin J. QuinonesKrishna Rao MaddipatiMiles Bergerfor the MADCO-PC Study TeamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niccolò Terrando John J. Park Michael Devinney Cliburn Chan Mary Cooter Pallavi Avasarala Joseph P. Mathew Quintin J. Quinones Krishna Rao Maddipati Miles Berger for the MADCO-PC Study Team Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
description	Abstract Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography–mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.
format	article
author	Niccolò Terrando John J. Park Michael Devinney Cliburn Chan Mary Cooter Pallavi Avasarala Joseph P. Mathew Quintin J. Quinones Krishna Rao Maddipati Miles Berger for the MADCO-PC Study Team
author_facet	Niccolò Terrando John J. Park Michael Devinney Cliburn Chan Mary Cooter Pallavi Avasarala Joseph P. Mathew Quintin J. Quinones Krishna Rao Maddipati Miles Berger for the MADCO-PC Study Team
author_sort	Niccolò Terrando
title	Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
title_short	Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
title_full	Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
title_fullStr	Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
title_full_unstemmed	Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
title_sort	immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4c6cc38806d44862bca6b02bf708d86d
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Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults

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