Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (<i>LPIN1</i>) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/4c6d2a5ff9f847128c065c04cea897cd |
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| Sumario: | Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (<i>LPIN1</i>) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation polymorphism analysis to investigate sequence variation in three regions of bovine <i>LPIN1</i> in New Zealand Holstein-Friesian × Jersey (HF × J)-cross dairy cows, including part of the 5′ non-coding region, the region containing the <i>LPIN1β</i>-spliced exon, and the sixth coding exon that encodes the putative transcriptional activating domain of the protein. No variation was found in the <i>LPIN1β</i>-spliced exon, but two sequence variants containing one single nucleotide polymorphism (SNP) were identified in the 5′ non-coding region and four sequence variants containing four non-synonymous SNPs were identified in the sixth coding exon. Among the three common variants of the sixth coding exon, variant <i>C</i> was found to be associated with an increase in milk fat percentage (presence 4.96 ± 0.034% vs. absence 4.81 ± 0.050%; <i>p</i> = 0.006) and milk protein percentage (presence 4.09 ± 0.017% vs. absence 3.99 ± 0.025%; <i>p</i> = 0.001), but no associations (<i>p</i> > 0.01) were detected for milk yield. These results suggest that variation in <i>LPIN1</i> affect the synthesis of fat and proteins in milk and has potential as a gene-marker to improve milk production traits. |
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