Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows

Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (<i>LPIN1</i>) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation...

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Autores principales:	Xiaohua Du, Huitong Zhou, Xia Liu, Yunhai Li, Jonathan G. H. Hickford
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>LPIN1</i> bovine milk fat percentage milk protein percentage PCR-SSCP Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/4c6d2a5ff9f847128c065c04cea897cd
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spelling	oai:doaj.org-article:4c6d2a5ff9f847128c065c04cea897cd2021-11-25T16:19:30ZSequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows10.3390/ani111132232076-2615https://doaj.org/article/4c6d2a5ff9f847128c065c04cea897cd2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3223https://doaj.org/toc/2076-2615Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (<i>LPIN1</i>) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation polymorphism analysis to investigate sequence variation in three regions of bovine <i>LPIN1</i> in New Zealand Holstein-Friesian × Jersey (HF × J)-cross dairy cows, including part of the 5′ non-coding region, the region containing the <i>LPIN1β</i>-spliced exon, and the sixth coding exon that encodes the putative transcriptional activating domain of the protein. No variation was found in the <i>LPIN1β</i>-spliced exon, but two sequence variants containing one single nucleotide polymorphism (SNP) were identified in the 5′ non-coding region and four sequence variants containing four non-synonymous SNPs were identified in the sixth coding exon. Among the three common variants of the sixth coding exon, variant <i>C</i> was found to be associated with an increase in milk fat percentage (presence 4.96 ± 0.034% vs. absence 4.81 ± 0.050%; <i>p</i> = 0.006) and milk protein percentage (presence 4.09 ± 0.017% vs. absence 3.99 ± 0.025%; <i>p</i> = 0.001), but no associations (<i>p</i> > 0.01) were detected for milk yield. These results suggest that variation in <i>LPIN1</i> affect the synthesis of fat and proteins in milk and has potential as a gene-marker to improve milk production traits.Xiaohua DuHuitong ZhouXia LiuYunhai LiJonathan G. H. HickfordMDPI AGarticle<i>LPIN1</i>bovinemilk fat percentagemilk protein percentagePCR-SSCPVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3223, p 3223 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>LPIN1</i> bovine milk fat percentage milk protein percentage PCR-SSCP Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	<i>LPIN1</i> bovine milk fat percentage milk protein percentage PCR-SSCP Veterinary medicine SF600-1100 Zoology QL1-991 Xiaohua Du Huitong Zhou Xia Liu Yunhai Li Jonathan G. H. Hickford Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
description	Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (<i>LPIN1</i>) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation polymorphism analysis to investigate sequence variation in three regions of bovine <i>LPIN1</i> in New Zealand Holstein-Friesian × Jersey (HF × J)-cross dairy cows, including part of the 5′ non-coding region, the region containing the <i>LPIN1β</i>-spliced exon, and the sixth coding exon that encodes the putative transcriptional activating domain of the protein. No variation was found in the <i>LPIN1β</i>-spliced exon, but two sequence variants containing one single nucleotide polymorphism (SNP) were identified in the 5′ non-coding region and four sequence variants containing four non-synonymous SNPs were identified in the sixth coding exon. Among the three common variants of the sixth coding exon, variant <i>C</i> was found to be associated with an increase in milk fat percentage (presence 4.96 ± 0.034% vs. absence 4.81 ± 0.050%; <i>p</i> = 0.006) and milk protein percentage (presence 4.09 ± 0.017% vs. absence 3.99 ± 0.025%; <i>p</i> = 0.001), but no associations (<i>p</i> > 0.01) were detected for milk yield. These results suggest that variation in <i>LPIN1</i> affect the synthesis of fat and proteins in milk and has potential as a gene-marker to improve milk production traits.
format	article
author	Xiaohua Du Huitong Zhou Xia Liu Yunhai Li Jonathan G. H. Hickford
author_facet	Xiaohua Du Huitong Zhou Xia Liu Yunhai Li Jonathan G. H. Hickford
author_sort	Xiaohua Du
title	Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
title_short	Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
title_full	Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
title_fullStr	Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
title_full_unstemmed	Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows
title_sort	sequence variation in the bovine lipin-1 gene (<i>lpin1</i>) and its association with milk fat and protein contents in new zealand holstein-friesian × jersey (hf × j)-cross dairy cows
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4c6d2a5ff9f847128c065c04cea897cd
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Sequence Variation in the Bovine Lipin-1 Gene (<i>LPIN1</i>) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows

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